Meghji S, Sandy J R, Scutt A M, Harvey W, Carter R L, Harris M
Joint Department of Oral and Maxillofacial Surgery, Institute of Dental Surgery and University College, London, UK.
Br J Cancer. 1988 Jul;58(1):17-21. doi: 10.1038/bjc.1988.153.
Three human cell lines derived from oro-pharyngeal squamous cell carcinomas of the head were investigated for bone-resorbing activity in vitro. Culture media from all three spontaneously produced a non-dialysable osteolytic factor with activity in three in vitro assays for interleukin 1 (IL1), viz. the lymphocyte activating factor (LAF) assay, stimulation of collagenase synthesis by articular chondrocytes, and stimulation of prostaglandin E2 synthesis by fibroblasts. Addition of anti-human IL1 antibody to the culture media abolished all the bone-resorbing activity. Fractionation of the cell culture media by high performance liquid chromatography (HPLC) showed a single peak of activity in the chondrocyte assay with an apparent mol.wt of 15-17,000. This co-eluted with activity in a preparation of IL1 from rat peritoneal macrophage cultures. These results indicate that IL1 is responsible for the prostaglandin-independent bone resorbing activity synthesised by these cells in vitro, and may contribute to the bone destruction associated with the tumour.
对源自头部口咽鳞状细胞癌的三种人类细胞系进行了体外骨吸收活性研究。所有三种细胞系的培养基均自发产生一种不可透析的溶骨因子，该因子在三种白细胞介素1（IL1）的体外测定中具有活性，即淋巴细胞激活因子（LAF）测定、关节软骨细胞对胶原酶合成的刺激以及成纤维细胞对前列腺素E2合成的刺激。向培养基中添加抗人IL1抗体可消除所有骨吸收活性。通过高效液相色谱（HPLC）对细胞培养基进行分级分离，在软骨细胞测定中显示出一个单一的活性峰，表观分子量为15 - 17,000。这与来自大鼠腹腔巨噬细胞培养物的IL1制剂中的活性共同洗脱。这些结果表明，IL1负责这些细胞在体外合成的与前列腺素无关的骨吸收活性，并且可能导致与肿瘤相关的骨破坏。
