临床耐甲氧西林凝固酶阴性葡萄球菌菌株中整合子和葡萄球菌盒式染色体类型的检测
Detection of Integrons and Staphylococcal Cassette Chromosome Types in Clinical Methicillin-resistant Coagulase Negative Staphylococci Strains.
作者信息
Hajiahmadi Fahimeh, Ghale Elham Salimi, Alikhani Mohammad Yousef, Mordadi Alireza, Arabestani Mohammad Reza
机构信息
Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.
Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
Osong Public Health Res Perspect. 2017 Feb;8(1):47-53. doi: 10.24171/j.phrp.2017.8.1.06. Epub 2017 Feb 28.
OBJECTIVES
Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance.
METHODS
Hundred clinical isolates of coagulase-negative staphylococci were characterized for integron content and staphylococcal cassette chromosome (SCCmec) type.
RESULTS
Sixteen isolates carried class 1 () integrons and four isolates carried class 2 () integrons. One resistance gene array was identified among the class 1 integrons ( cassette). The distribution of SCCmec types in 50 methicillin-resistant coagulase-negative staphylococci strains showed that SCCmec types III and V dominated among the tested strains.
CONCLUSION
This is the first report of methicillin-resistant coagulase-negative staphylococci strains that carry two mobile genetic elements, including class 1 and 2 integrons and SCCmec, in Iran.
目的
整合子被认为在抗生素耐药性传播中起重要作用。本研究调查了在伊朗分离出的1类和2类整合子阳性的耐甲氧西林凝固酶阴性葡萄球菌菌株，并对其耐药模式进行了表征。
方法
对100株凝固酶阴性葡萄球菌临床分离株进行整合子含量和葡萄球菌盒式染色体（SCCmec）类型鉴定。
结果
16株携带1类（）整合子，4株携带2类（）整合子。在1类整合子中鉴定出一个耐药基因阵列（盒式）。50株耐甲氧西林凝固酶阴性葡萄球菌菌株中SCCmec类型的分布表明，III型和V型SCCmec在受试菌株中占主导地位。
结论
这是伊朗首次报道携带1类和2类整合子以及SCCmec这两种移动遗传元件的耐甲氧西林凝固酶阴性葡萄球菌菌株。
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