Moshiri Mona, Dallal Mohammad Mehdi Soltan, Rezaei Farhad, Douraghi Masoumeh, Sharifi Laleh, Noroozbabaei Zahra, Gholami Mehrdad, Mirshafiey Abbas
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Osong Public Health Res Perspect. 2017 Feb;8(1):54-60. doi: 10.24171/j.phrp.2017.8.1.07. Epub 2017 Feb 28.
Gastrointestinal disorders caused by serovar Enteritidis (sE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards sE. The purpose of this work was to assess the effect of PTCC 1643 on cultured intestinal epithelial cells infected with sE.
HT29 cells were cultured in Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The cells were treated with PTCC 1643 after or before challenge with sE. At 2 and 4 hours post-infection, we measured changes in the expression levels of and via real-time polymerase chain reaction.
Treatment with inhibited sE-induced increases in and expression in the infected HT29 cells. Moreover, the expression of and in cells that were pretreated with and then infected with sE was significantly higher than that in cells infected with sE without pretreatment. Taken together, the results indicated that had an anti-inflammatory effect and modulated the innate immune response to sE by influencing and expression.
Our findings suggested that PTCC 1643 was able to suppress inflammation caused by sE infection in HT29 cells and reduce and expression. Additional in vivo and in vitro studies are required to further elucidate the mechanisms underlying this anti-inflammatory effect.
肠炎血清型（sE）引起的胃肠道疾病是全球范围内的一个重大健康问题。益生菌已被证明对免疫反应有积极影响。研究了其影响HT29肠上皮细胞对sE的固有免疫反应的能力。这项工作的目的是评估PTCC 1643对感染sE的培养肠上皮细胞的影响。
HT29细胞在补充有10%胎牛血清和1%青霉素/链霉素的罗斯威尔公园纪念研究所培养基中培养。在用sE攻击之前或之后用PTCC 1643处理细胞。在感染后2小时和4小时，我们通过实时聚合酶链反应测量了[具体基因名称未给出]和[具体基因名称未给出]表达水平的变化。
用[具体物质名称未给出]处理可抑制感染的HT29细胞中sE诱导的[具体基因名称未给出]和[具体基因名称未给出]表达增加。此外，先用[具体物质名称未给出]预处理然后感染sE的细胞中[具体基因名称未给出]和[具体基因名称未给出]的表达明显高于未预处理而感染sE的细胞。综上所述，结果表明[具体物质名称未给出]具有抗炎作用，并通过影响[具体基因名称未给出]和[具体基因名称未给出]的表达来调节对sE的固有免疫反应。
我们的研究结果表明PTCC 1643能够抑制HT29细胞中sE感染引起的炎症并降低[具体基因名称未给出]和[具体基因名称未给出]的表达。需要进一步的体内和体外研究来阐明这种抗炎作用的潜在机制。
