Matsumoto E, Muragaki Y, Ooshima A, Terasawa M, Imayoshi T
Department of Pathology, Wakayama Medical College, Japan.
Exp Mol Pathol. 1988 Oct;49(2):243-53. doi: 10.1016/0014-4800(88)90037-8.
The authors developed a competitive enzyme immunoassay for serum immunoreactive prolyl hydroxylase (SIRPH) as a marker of fibrogenesis, and examined the changes in SIRPH concentrations in rats with carrageenan-induced granuloma and adjuvant arthritis. The effects of such anti-inflammatory agents as prednisolone, pranoprofen, indomethacin, and hydrocortisone were also investigated. Prolyl hydroxylase activity in rats with carrageenan-induced granuloma and adjuvant arthritis increased inflammatory granulation tissue, and the concentrations of SIRPH also increased time dependently. The nontreated controls showed a constant low level of SIRPH. After treatment with anti-inflammatory agents or removal of the granuloma, SIRPH levels decreased coincident with the improvement of clinical symptoms. It was assumed that immunoreactive prolyl hydroxylase was released into the blood stream as a result of increased turnover of the enzyme protein owing to fibrotic disorders. SIRPH could be a useful biochemical marker for assessing therapeutic effects through the actual activity of fibrogenesis.
作者开发了一种竞争性酶免疫测定法，用于检测血清免疫反应性脯氨酰羟化酶（SIRPH），作为纤维生成的标志物，并研究了角叉菜胶诱导的肉芽肿和佐剂性关节炎大鼠中SIRPH浓度的变化。还研究了泼尼松龙、普拉洛芬、吲哚美辛和氢化可的松等抗炎药的作用。角叉菜胶诱导的肉芽肿和佐剂性关节炎大鼠的脯氨酰羟化酶活性增加了炎性肉芽组织，SIRPH的浓度也随时间依赖性增加。未治疗的对照组SIRPH水平持续较低。用抗炎药治疗或切除肉芽肿后，SIRPH水平随着临床症状的改善而下降。据推测，由于纤维化疾病导致酶蛋白周转增加，免疫反应性脯氨酰羟化酶释放到血流中。SIRPH可能是一种通过纤维生成的实际活性评估治疗效果的有用生化标志物。
