School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-744, Korea.
Lab Chip. 2017 May 16;17(10):1817-1825. doi: 10.1039/c7lc00363c.
There are many proposed mechanisms by which single cells can be trapped; among them is the through-hole membrane for the characterization of individual microorganisms. Due to the small scale of the fabricated pores, the construction of through-hole membranes on a large scale and with relatively large areas faces many difficulties. This paper describes novel fabrication methods for a large-area, freestanding micro/nano through-hole membrane constructed from versatile membrane materials using through-hole membranes on a microfluidic chip (THMMC). This process can rapidly (<20 min) fabricate membranes with high fidelity multiscale hole size without residual layers. The through-hole site was easily customizable from the micro to the nanoscale, with a low or high aspect ratio giving rise to reliable membranes. Also, the rigidity and biocompatibility of the through-hole membrane are easily tunable by simple injection of versatile membrane materials to obtain a large area (up to 3600 mm). Membranes produced in this manner were then applied as a proof of concept for the isolation, cultivation, and quantification of individual micro-algal cells for selection with respect to the growth rate, while controlling the quorum sensing mediated metabolic and proliferative changes.
有许多提出的机制可以捕获单细胞;其中包括用于单个微生物表征的通孔膜。由于制造的孔的小尺寸，在大规模和相对大面积上构建通孔膜面临许多困难。本文描述了一种新颖的制造方法，用于使用微流控芯片上的通孔膜 (THMMC) 从多功能膜材料构建大面积、独立式微/纳米通孔膜。这个过程可以快速 (<20 分钟) 制造具有高保真度多尺度孔尺寸的膜，而没有残留层。通孔位置很容易从微观到纳米尺度进行定制，具有低或高纵横比，从而产生可靠的膜。此外，通过简单地注入多功能膜材料，很容易调整通孔膜的刚性和生物相容性，从而获得大面积 (高达 3600 毫米)。以这种方式制造的膜随后被用作概念验证，用于分离、培养和量化单个微藻细胞，以根据生长速度进行选择，同时控制群体感应介导的代谢和增殖变化。
