Biomaterials Science and Technology, Universiteit Twente Faculteit Technische Natuurwetenschappen, Enschede, The Netherlands
Biomaterials Science and Technology, Universiteit Twente Faculteit Technische Natuurwetenschappen, Enschede, The Netherlands.
J R Soc Interface. 2018 Jul;15(144). doi: 10.1098/rsif.2018.0351.
In recent years, organs-on-chips (OOCs) have been developed to meet the desire for more realistic cell culture models. These systems introduce microfluidics, mechanical stretch and other physiological stimuli to models, thereby significantly enhancing their descriptive power. In most OOCs, porous polymeric membranes are used as substrates for cell culture. The polymeric material, morphology and shape of these membranes are often suboptimal, despite their importance for achieving ideal cell functionality such as cell-cell interaction and differentiation. The currently used membranes are flat and thus do not account for the shape and surface morphology of a tissue. Moreover, the polymers used for fabrication of these membranes often lack relevant characteristics, such as mechanical properties matching the tissue to be developed and/or cytocompatibility. Recently, innovative techniques have been reported for fabrication of porous membranes with suitable porosity, shape and surface morphology matching the requirements of OOCs. In this paper, we review the state of the art for developing these membranes and discuss their application in OOCs.
近年来，器官芯片（OOCs）的发展满足了人们对更真实的细胞培养模型的渴望。这些系统引入了微流控、机械拉伸和其他生理刺激，从而显著提高了模型的描述能力。在大多数 OOCs 中，多孔聚合物膜被用作细胞培养的基底。尽管这些膜对于实现理想的细胞功能（如细胞-细胞相互作用和分化）非常重要，但它们的聚合物材料、形态和形状往往并不理想。目前使用的膜是平面的，因此不能考虑组织的形状和表面形态。此外，用于制造这些膜的聚合物通常缺乏相关特性，例如与要开发的组织匹配的机械性能和/或细胞相容性。最近，有报道称采用了一些创新技术来制造具有合适孔隙率、形状和表面形态的多孔膜，以满足 OOCs 的要求。本文综述了开发这些膜的最新进展，并讨论了它们在 OOCs 中的应用。
