Keeney M, Hedley B D, Chin-Yee I H
Pathology and Laboratory Medicine, Department of Hematology, London Health Sciences Centre, Victoria Hospital, London, ON, Canada.
Department of Medicine, Schulich School of Medicine Western University, London, ON, Canada.
Int J Lab Hematol. 2017 May;39 Suppl 1:86-92. doi: 10.1111/ijlh.12666.
Flow cytometry is an invaluable technology in the examination of blood, bone marrow, tissue and body fluids for the presence or absence of hematological disease. It is used in both diagnostic and follow-up testing, with an increasingly important role in the detection of very small residual disease populations (Minimal Residual Disease, MRD) However, flow cytometry immunophenotyping of leukemia and lymphoma is highly dependent on interpretation of results and with the increased complexity of 8-10 color instruments routinely used in clinical laboratories, knowledge of disease-defining populations is increasingly important as is recognizing normal and reactive patterns. This manuscript presents case studies with flow cytometric patterns encountered in routine screening of samples sent for leukemia and lymphoma immunophenotyping, focusing mainly on B-cell disorders which may be missed or incorrectly interpreted by the laboratory (including a hematopathologist) performing the test. Case studies are used to illustrate our laboratory's standardized approach to the interpretation of flow cytometric data. In addition to a standardized approach, these cases emphasize the importance of interpretative skills of technologist and hematopathologists in recognizing abnormal patterns in detecting hematological malignancies.
流式细胞术是一种非常重要的技术，可用于检查血液、骨髓、组织和体液中是否存在血液系统疾病。它用于诊断和随访检测，在检测极小残留病群体（微小残留病，MRD）方面发挥着越来越重要的作用。然而，白血病和淋巴瘤的流式细胞术免疫表型分析高度依赖于结果的解读，随着临床实验室常规使用的8-10色仪器的复杂性增加，了解疾病定义群体变得越来越重要，识别正常和反应性模式也是如此。本文介绍了在白血病和淋巴瘤免疫表型分析送检样本的常规筛查中遇到的流式细胞术模式的案例研究，主要关注可能被进行检测的实验室（包括血液病理学家）遗漏或错误解读的B细胞疾病。案例研究用于说明我们实验室对流式细胞术数据解读的标准化方法。除了标准化方法外，这些案例强调了技术人员和血液病理学家在识别血液系统恶性肿瘤异常模式方面的解读技能的重要性。
