Key Laboratory of Biodiversity Conservation in Southwest China, the State Forest Administration, Southwest Forestry University, Kunming, Yunnan Province, China, 650224.
Central Laboratory of Xiangyang No.1 Hospital, College of Basic Medical Sciences, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan, Hubei Province, China, 442000.
FEMS Yeast Res. 2017 May 1;17(3). doi: 10.1093/femsyr/fox023.
Saccharomyces uvarum is a good wine yeast species that may have great potential for the future. However, sulfur tolerance of most S. uvarum strains is very poor. In addition there is still little information about the SSU1 gene of S. uvarum, which encodes a putative transporter conferring sulfite tolerance. In order to analyze the function of the SSU1 gene, two expression vectors that contained different SSU1 genes were constructed and transferred into a sulfite-tolerant S. uvarum strain, A9. Then sulfite tolerance, SO2 production, and PCR, sequencing, RT-qPCR and transcriptome analyses were used to access the function of the S. uvarum SSU1 gene. Our results illustrated that enhancing expression of the SSU1 gene can promote sulfite resistance in S. uvarum, and an insertion fragment ahead of the additional SSU1 gene, as seen in some alleles, could affect the expression of other genes and the sulfite tolerance level of S. uvarum. This is the first report on enhancing the expression of the SSU1 gene of S. uvarum.
酒香酵母是一种优良的酿酒酵母，未来可能具有巨大的潜力。然而，大多数酒香酵母菌株的耐硫性很差。此外，关于编码亚硫酸盐耐受的假定转运蛋白的 SSU1 基因，仍然知之甚少。为了分析 SSU1 基因的功能，构建了两个含有不同 SSU1 基因的表达载体，并将其转入耐亚硫酸盐的酒香酵母菌株 A9 中。然后通过亚硫酸盐耐受性、SO2 产量以及 PCR、测序、RT-qPCR 和转录组分析来评估 S. uvarum SSU1 基因的功能。我们的结果表明，增强 SSU1 基因的表达可以促进酒香酵母对亚硫酸盐的抗性，并且在某些等位基因中看到的在额外的 SSU1 基因之前的插入片段可能会影响其他基因的表达和酒香酵母的亚硫酸盐耐受水平。这是关于增强 S. uvarum SSU1 基因表达的首次报道。
