Berkovitch Yulia, Seliktar Dror
The Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel; The Interdisciplinary Program for Biotechnology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
The Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Int J Pharm. 2017 May 25;523(2):545-555. doi: 10.1016/j.ijpharm.2016.11.032.
This study describes the use of a set of protein-based biomaterials that allow us to explore the mechanism of cell-mediated 3-D invasion associated with peripheral nerve regeneration. Hydrogels made from poly(ethylene glycol) (PEG) conjugated extracellular matrix proteins, including fibrinogen, gelatin and albumin were compared in their ability to support the neurite extension and glial cell migration from dorsal root ganglion (DRG) as compared to PEG only hydrogel controls. The synthetic polymer in the system provides a cross-linked network with controlled mechanical properties and degradation, whereas the protein components provide the unique extracellular matrix (ECM) for controlling neuronal cell morphogenesis. A range of hydrogel compositions were found to support DRG cell outgrowth, based on the mechanical properties, density and proteolytic degradation of the matrix. The 3-D invasion and morphogenesis of newly grown neurites and glial cells in the different materials were characterized and correlated to the properties of the scaffolds. The DRG cell outgrowth was highly correlated with the density of different hydrogel compositions.
本研究描述了一组基于蛋白质的生物材料的应用，这些材料使我们能够探索与周围神经再生相关的细胞介导的三维侵袭机制。将由聚乙二醇（PEG）共轭细胞外基质蛋白制成的水凝胶，包括纤维蛋白原、明胶和白蛋白，与仅含PEG的水凝胶对照相比，比较它们支持背根神经节（DRG）神经突延伸和胶质细胞迁移的能力。系统中的合成聚合物提供具有可控机械性能和降解的交联网络，而蛋白质成分提供用于控制神经元细胞形态发生的独特细胞外基质（ECM）。基于基质的机械性能、密度和蛋白水解降解，发现一系列水凝胶组合物支持DRG细胞生长。对不同材料中新生长的神经突和胶质细胞的三维侵袭和形态发生进行了表征，并与支架的特性相关联。DRG细胞生长与不同水凝胶组合物的密度高度相关。
