超高压处理提高了蘑菇脚蛋白的体外酶解效率和具有激活酒精代谢酶活性的生物活性肽的释放。
Ultra-high-pressure processing improves proteolysis and release of bioactive peptides with activation activities on alcohol metabolic enzymes in vitro from mushroom foot protein.
机构信息
Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083 Beijing, People's Republic of China; Beijing Engineering Research Center of Protein & Functional Peptides, China National Research Institute of Food & Fermentation Industries, 100015 Beijing, People's Republic of China.
Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083 Beijing, People's Republic of China.
出版信息
Food Chem. 2017 Sep 15;231:25-32. doi: 10.1016/j.foodchem.2017.03.058. Epub 2017 Mar 14.
This study was to find an effective process to extract bioactive peptides from mushroom foot and determine their effects on activation of alcohol metabolic enzymes in vitro. The optimum extraction assisted by ultra-high-pressure processing of mushroom foot peptides was obtained with a pressure of 400MPa and a processing time of 10min. After ultrafiltration, peptides with molecular weight of 0-3kDa had the highest activity to activate alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) by 70.79% and 71.35%, respectively. Following dextran gel chromatography, two peaks (p-I and p-II) appeared and the activation activities on ADH and ALDH of p-I were 72.00% and 73.43%, both higher than p-II. Nine peptides were found in p-I as determined by LC-MS/MS, and two of them (IPLH and IPIVLL) were synthesized. IPLH activated ADH and ALDH by 42.7% and 29.2% respectively, which were higher than IPIVLL.
本研究旨在寻找一种从蘑菇脚中提取生物活性肽的有效方法，并确定其对体外酒精代谢酶激活的影响。采用超高压辅助提取蘑菇脚肽，最佳提取条件为压力 400MPa，处理时间 10min。超滤后，分子量为 0-3kDa 的肽对醇脱氢酶（ADH）和醛脱氢酶（ALDH）的激活活性最高，分别为 70.79%和 71.35%。经葡聚糖凝胶层析后，出现两个峰（p-I 和 p-II），p-I 对 ADH 和 ALDH 的激活活性分别为 72.00%和 73.43%，均高于 p-II。通过 LC-MS/MS 确定 p-I 中存在 9 种肽，其中两种肽（IPLH 和 IPIVLL）被合成。IPLH 对 ADH 和 ALDH 的激活活性分别为 42.7%和 29.2%，均高于 IPIVLL。
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