Jordens J Z, Hall L M
Department of Medical Microbiology, London Hospital Medical College.
J Med Microbiol. 1988 Oct;27(2):117-23. doi: 10.1099/00222615-27-2-117.
Clinical isolates of Staphylococcus aureus from the London Hospital were characterised by genetic analysis of antibiotic-resistance determinants and by restriction endonuclease digestion of chromosomal DNA and compared with isolates from elsewhere in the UK. Restriction enzyme digestion of chromosomal DNA confirmed that a single strain of methicillin-resistant S. aureus (MRSA) persists at the London Hospital, although its antibiotic-resistance profile and plasmid carriage are not constant. Methicillin-sensitive isolates, on the other hand, each had readily distinguishable and unique DNA restriction patterns. The DNA restriction digest pattern of the London Hospital MRSA isolates was identical to that of "epidemic" (E) MRSA isolates from the Thames regions. By contrast, other MRSA isolates had DNA restriction patterns which differed from those of EMRSA isolates and from each other. These results confirm the discriminatory value of restriction pattern analysis as a typing method.
对伦敦医院的金黄色葡萄球菌临床分离株进行了抗生素抗性决定因素的基因分析以及染色体DNA的限制性内切酶消化分析，并与英国其他地方的分离株进行了比较。染色体DNA的限制性酶切证实，耐甲氧西林金黄色葡萄球菌（MRSA）的单一菌株在伦敦医院持续存在，尽管其抗生素抗性谱和质粒携带情况并不恒定。另一方面，甲氧西林敏感分离株各自具有易于区分的独特DNA限制性图谱。伦敦医院MRSA分离株的DNA限制性酶切图谱与泰晤士地区“流行”（E）MRSA分离株的图谱相同。相比之下，其他MRSA分离株的DNA限制性图谱与EMRSA分离株的图谱不同，且彼此之间也不同。这些结果证实了限制性图谱分析作为一种分型方法的鉴别价值。
