Yang Qianqian, Hou Chen, Huang Da, Zhuang Chunbo, Jiang Weichao, Geng Zhi, Wang Xiaobei, Hu Lihua
Department of Clinical Laboratory, Union Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
Department of Blood Transfusion, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Oncol Lett. 2017 Mar;13(3):1958-1964. doi: 10.3892/ol.2017.5608. Epub 2017 Jan 17.
Although there is evidence that galectin-9 is a critical factor in health and disease, the upstream regulatory microRNA (miRNA or miR) of the protein remains poorly defined. miR-455-5p is characterized as a tumor-associated miRNA in cancer research. However, the actual role of miR-455-5p with respect to inhibiting or promoting tumorigenesis in colon cancer is unclear. The present study aimed to investigate the expression, role and target regulation association of galectin-9 and miR-455-5p in colon cancer. Western blot analysis and reverse transcription-quantitative polymerase chain reaction were used for the detection of the expression levels of galectin-9 and miRNAs. Cell Counting kit-8 test was used for the evaluation of cell proliferation, while flow cytometry was used for cell apoptosis analysis. A potential interaction between galectin-9 and miR-455-5p was predicted by target prediction programs and confirmed by luciferase assay and transfection with miRNA mimics. The present study revealed that elevated expression of galectin-9 and miR-455-5p in colon cancer was associated with HT29 cell proliferation and apoptosis. Furthermore, the present study demonstrated that miR-455-5p reduced galectin-9 expression by directly targeting its 3'-untranslated region. These data suggest that miR-455-5p functions as a potential oncogene in colon cancer by targeting galectin-9.
尽管有证据表明半乳糖凝集素-9是健康和疾病中的关键因素，但该蛋白的上游调控微小RNA（miRNA或miR）仍不清楚。在癌症研究中，miR-455-5p被表征为一种肿瘤相关miRNA。然而，miR-455-5p在结肠癌中抑制或促进肿瘤发生方面的实际作用尚不清楚。本研究旨在探讨半乳糖凝集素-9和miR-455-5p在结肠癌中的表达、作用及靶标调控关系。采用蛋白质印迹分析和逆转录-定量聚合酶链反应检测半乳糖凝集素-9和miRNA的表达水平。使用细胞计数试剂盒-8检测评估细胞增殖，同时采用流式细胞术进行细胞凋亡分析。通过靶标预测程序预测半乳糖凝集素-9和miR-455-5p之间的潜在相互作用，并通过荧光素酶测定和miRNA模拟物转染进行验证。本研究表明，结肠癌中半乳糖凝集素-9和miR-455-5p的表达升高与HT29细胞增殖和凋亡有关。此外，本研究证明miR-455-5p通过直接靶向半乳糖凝集素-9的3'-非翻译区降低其表达。这些数据表明，miR-455-5p通过靶向半乳糖凝集素-9在结肠癌中发挥潜在癌基因的作用。
