Alikhani Pour Mahdi, Sardari Soroush, Eslamifar Ali, Azhar Abid, Rezvani Mohammad, Nazari Milad
Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 13164, Iran.
Department of Clinical Research, Pasteur Institute of Iran, Tehran 13164, Iran.
Iran Biomed J. 2017 Nov;21(6):400-5. doi: 10.18869/acadpub.ibj.21.6.400. Epub 2017 Apr 29.
Medicinal plants, as a complementary medicine, have been used to treat various diseases since ancient times. These plants have numerous beneficial applications and are the source of certain conventional drugs. In diseases such as stroke and ischemia, which are caused by several factors, abnormal coagulation is an important causative factor. Accordingly, novel and effective therapies such as herbal remedies should be practiced to prevent such lethal diseases.
Using the available databases such as Google Scholar and PubMed, the previously reported anticoagulant compounds and plants possessing anticoagulant activity were identified and collected in two separate lists. Next, the fast and cost-effective cheminformatics methods incorporated in PubChem were applied to detect some compounds similar to reported anticoagulants. Subsequently, 15 native medical plants of Iran containing the potential anticoagulants were selected. The selected plants were purchased and chopped, and the potential compounds were extracted by ethanol. Then three concentrations of extracts (1, 10, and 100 µg per ml) were made. Finally, anticoagulant effect of the selected plants was evaluated by in vitro prothrombin time and activated partial thromboplastin time coagulation tests.
Among the 15 selected medicinal plants, three plants, including Terminalia bellirica (P=0.0019), Astragalus arbusculinus (P=0.0021), and Origanum vulgare (P=0.0014) showed a more promising anticoagulant effect in comparison to the control.
The anticoagulant activity was identified for the first time in these three plants. Further in vivo study and mechanism of action assay are required to be performed on these three plants, which could be suitable candidates for use as natural anticoagulant medicines.
药用植物作为一种补充药物，自古以来就被用于治疗各种疾病。这些植物有许多有益的应用，并且是某些传统药物的来源。在由多种因素引起的中风和缺血等疾病中，异常凝血是一个重要的致病因素。因此，应该采用诸如草药疗法等新颖有效的治疗方法来预防此类致命疾病。
利用谷歌学术和PubMed等现有数据库，在两个单独的列表中识别并收集先前报道的具有抗凝活性的化合物和植物。接下来，应用PubChem中包含的快速且经济高效的化学信息学方法来检测一些与报道的抗凝剂相似的化合物。随后，选择了15种含有潜在抗凝剂的伊朗本土药用植物。购买所选植物并切碎，用乙醇提取潜在化合物。然后制备三种提取物浓度（每毫升1、10和100微克）。最后，通过体外凝血酶原时间和活化部分凝血活酶时间凝血试验评估所选植物的抗凝效果。
在15种所选药用植物中，三种植物，包括毛诃子（P = 0.0019）、丛生黄芪（P = 0.0021）和牛至（P = 0.0014）与对照组相比显示出更有前景的抗凝效果。
首次在这三种植物中鉴定出抗凝活性。需要对这三种植物进行进一步的体内研究和作用机制分析，它们可能是用作天然抗凝血药物的合适候选者。
