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在土耳其艾登市美发行业从业者中使用口腔微核试验进行遗传毒性风险评估。

Genotoxic risk assessment in professionals working hairdressers area using buccal micronucleus assay, in Aydın City, Turkey.

作者信息

Aslantürk Özlem Sultan, Aşkin Çelik Tülay

机构信息

Faculty of Art and Science, Department of Biology, Adnan Menderes University, 09010, Aydın, Turkey.

出版信息

Environ Sci Pollut Res Int. 2017 Jun;24(17):14700-14705. doi: 10.1007/s11356-017-9075-5. Epub 2017 Apr 29.

Abstract

The aim of this study was to determine the genotoxic risk of professional hairdressers in Aydın City, Turkey, through investigating the micronucleus frequencies in buccal mucosa epithelial cells. All the hairdresser working hairdresser area were included in the genotoxic risk group (GRG = 20) in Aydın City, Turkey. The control group (CG = 20) comprised healthy individuals matching the gender and age of the GRG. Buccal mucosal scraping from all the 40 subjects of GRG (10 women and 10 men) and CG (10 women and 10 men) was stained with Giemsa stain and observed under light microscope (×40) for the presence of micronuclei (M 10 N) and karyolysis, pyknosis, condensed chromatin, karyorrhexis, nuclear bud, and binucleates in the exfoliated epithelial cells. There are significance between the incidence of MN in GRG and CG (P = <0.005) using one-way ANOVA, Kolmogorov-Smirnov Z test, and Spearman Rank Correlation Tests.

摘要

本研究的目的是通过调查颊黏膜上皮细胞中的微核频率,确定土耳其艾登市职业美发师的遗传毒性风险。土耳其艾登市所有在美发工作区域工作的美发师都被纳入遗传毒性风险组(GRG = 20)。对照组(CG = 20)由与遗传毒性风险组性别和年龄匹配的健康个体组成。对遗传毒性风险组(10名女性和10名男性)和对照组(10名女性和10名男性)共40名受试者的颊黏膜刮片进行吉姆萨染色,并在光学显微镜(×40)下观察脱落上皮细胞中微核(M 10 N)以及核溶解、核固缩、染色质凝聚、核碎裂、核芽和双核的存在情况。使用单因素方差分析、柯尔莫哥洛夫-斯米尔诺夫Z检验和斯皮尔曼等级相关检验,遗传毒性风险组和对照组的微核发生率之间存在显著差异(P = <0.005)。

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