Luo Dingyuan, Chen Wei, Tian Yun, Li Junliang, Xu Xinke, Chen Cheng, Li Fangcheng
Department of Vascular and Thyroid Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University.
Department of Neurosurgery, Guangzhou Women and Children's Medical Center.
Onco Targets Ther. 2017 Apr 18;10:2173-2181. doi: 10.2147/OTT.S133022. eCollection 2017.
Glioma is the most common and aggressive human primary tumor in the central nervous system. Despite present clinical advancements, median survival time remains poor in this malignant tumor. Serpin peptidase inhibitor, clade A member 3 (SERPINA3), is a member of the serpin superfamily of protease inhibitors. Its aberrant expression has been observed in various tumors. However, its clinical significance and biological function in glioma remain unclear, especially for the prognosis of glioma patients. In this study, we investigated SERPINA3 expression in glioma tissue samples and its significance in predicting the prognosis of glioma patients. SERPINA3 protein expression was studied by immunohistochemistry, while real-time polymerase chain reaction was used to study SERPINA3 mRNA expression. We found that SERPINA3 was upregulated in glioma tissue at both mRNA and protein levels, compared with noncancerous brain tissues. We also found that high SERPINA3 expression in glioma tissues correlated significantly with advanced World Health Organization grade. Univariate and multivariate analyses revealed that high SERPINA3 expression was an independent prognostic factor for poor overall survival of glioma patients. Moreover, our findings were further validated by online Oncomine database. Taken together, our results suggest that SERPINA3 plays an oncogenic role in glioma progression and provide an insight into the application of SERPINA3 as a novel predictor of clinical outcomes and a potential biomarker of glioma.
胶质瘤是中枢神经系统中最常见且侵袭性最强的人类原发性肿瘤。尽管目前临床有了进展，但这种恶性肿瘤的中位生存时间仍然较短。丝氨酸蛋白酶抑制剂A3家族成员（SERPINA3）是丝氨酸蛋白酶抑制剂超家族的一员。其异常表达已在多种肿瘤中被观察到。然而，其在胶质瘤中的临床意义和生物学功能仍不清楚，尤其是对胶质瘤患者预后的影响。在本研究中，我们调查了SERPINA3在胶质瘤组织样本中的表达及其在预测胶质瘤患者预后方面的意义。通过免疫组织化学研究SERPINA3蛋白表达，同时使用实时聚合酶链反应研究SERPINA3 mRNA表达。我们发现，与非癌脑组织相比，SERPINA3在胶质瘤组织中的mRNA和蛋白水平均上调。我们还发现，胶质瘤组织中SERPINA3的高表达与世界卫生组织高级别显著相关。单因素和多因素分析显示，SERPINA3高表达是胶质瘤患者总体生存不良的独立预后因素。此外，我们的研究结果通过在线Oncomine数据库得到了进一步验证。综上所述，我们的结果表明SERPINA3在胶质瘤进展中起致癌作用，并为将SERPINA3作为临床结局的新型预测指标和胶质瘤潜在生物标志物的应用提供了见解。
