Hebbel Robert P
J Clin Invest. 2017 May 1;127(5):1613-1615. doi: 10.1172/JCI93649.
In the mid-1990s, my research group began to devise a method to establish endothelial cell cultures from human peripheral blood, with an ultimate goal of examining interindividual heterogeneity of endothelial biology. The initial work, published in the JCI in 2000, described the method enabling successful attainment of blood outgrowth endothelial cells (BOEC). Truly endothelial, BOEC are progeny of a transplantable cell that originates in bone marrow, a putative endothelial progenitor. Our subsequent experimental work focused upon practical applications of BOEC: their use for gene therapy, tissue engineering, assessment of mutant gene effect, and discovery of heterogeneity in endothelial biology.
20世纪90年代中期，我的研究团队开始设计一种从人外周血中建立内皮细胞培养物的方法，最终目标是研究内皮生物学的个体间异质性。2000年发表在《临床研究杂志》（JCI）上的初步研究成果描述了一种成功获得血源性内皮细胞（BOEC）的方法。BOEC是真正的内皮细胞，是一种起源于骨髓的可移植细胞（一种假定的内皮祖细胞）的后代。我们随后的实验工作聚焦于BOEC的实际应用：将其用于基因治疗、组织工程、突变基因效应评估以及内皮生物学异质性的发现。
