de Wit Emmie, Rosenke Kyle, Fischer Robert J, Marzi Andrea, Prescott Joseph, Bushmaker Trenton, van Doremalen Neeltje, Emery Shannon L, Falzarano Darryl, Feldmann Friederike, Groseth Allison, Hoenen Thomas, Juma Bonventure, McNally Kristin L, Ochieng Melvin, Omballa Victor, Onyango Clayton O, Owuor Collins, Rowe Thomas, Safronetz David, Self Joshua, Williamson Brandi N, Zemtsova Galina, Grolla Allen, Kobinger Gary, Rayfield Mark, Ströher Ute, Strong James E, Best Sonja M, Ebihara Hideki, Zoon Kathryn C, Nichol Stuart T, Nyenswah Tolbert G, Bolay Fatorma K, Massaquoi Moses, Feldmann Heinz, Fields Barry
Laboratory of Virology.
Centers for Disease Control and Prevention, Atlanta, Georgia.
J Infect Dis. 2016 Oct 15;214(suppl 3):S169-S176. doi: 10.1093/infdis/jiw216. Epub 2016 Jun 21.
West Africa experienced the first epidemic of Ebola virus infection, with by far the greatest number of cases in Guinea, Sierra Leone, and Liberia. The unprecedented epidemic triggered an unparalleled response, including the deployment of multiple Ebola treatment units and mobile/field diagnostic laboratories. The National Institute of Allergy and Infectious Diseases and the Centers for Disease Control and Prevention deployed a joint laboratory to Monrovia, Liberia, in August 2014 to support the newly founded Ebola treatment unit at the Eternal Love Winning Africa (ELWA) campus. The laboratory operated initially out of a tent structure but quickly moved into a fixed-wall building owing to severe weather conditions, the need for increased security, and the high sample volume. Until May 2015, when the laboratory closed, the site handled close to 6000 clinical specimens for Ebola virus diagnosis and supported the medical staff in case patient management. Laboratory operation and safety, as well as Ebola virus diagnostic assays, are described and discussed; in addition, lessons learned for future deployments are reviewed.
西非经历了首次埃博拉病毒感染疫情,几内亚、塞拉利昂和利比里亚的病例数迄今为止最多。这场前所未有的疫情引发了史无前例的应对行动,包括部署多个埃博拉治疗单元以及移动/现场诊断实验室。美国国家过敏和传染病研究所及疾病控制与预防中心于2014年8月在利比里亚蒙罗维亚部署了一个联合实验室,以支持在永恒之爱胜利非洲(ELWA)校区新建的埃博拉治疗单元。该实验室最初在帐篷结构中运作,但由于恶劣天气条件、对加强安保的需求以及高样本量,很快就搬到了有固定墙壁的建筑中。直到2015年5月实验室关闭,该地点处理了近6000份用于埃博拉病毒诊断的临床标本,并在病例患者管理方面为医务人员提供了支持。本文描述并讨论了实验室操作与安全以及埃博拉病毒诊断检测方法;此外,还回顾了未来部署中吸取的经验教训。