Lim Chae Jeong, Yoon Yeo Sung, Ryu Pan Dong
Department of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Anatomy & Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
J Acupunct Meridian Stud. 2020 Feb;13(1):33-38. doi: 10.1016/j.jams.2019.11.002. Epub 2019 Nov 16.
The primo vascular system (PVS) is reported to have a periductium composed of cells with spherical or spindle-shaped nuclei and abundant cytoplasm. However, little is known about these periductium cells. In this study, we examined the morphological features of cells covering the PVS tissue isolated from the surface of abdominal organs of rats. By hematoxylin and eosin (H&E) staining, we observed a layer of dark nuclei on the basement membrane at the borders of the sections of primo node (PN), primo vessel (PV), and their subunits. The nuclei appeared thin and linear (10-14 μm), elliptical (8-10 × 3-4 μm), and round (5-7 μm). The borders of the PVS tissue sections were immunostained with a selective antibody for mesothelial cells (MCs). Areas of immunoreactivity overlapped with the flattened cells are shown by hematoxylin and eosin staining. By scanning electron microscopy, we further identified elliptical (11 × 21 μm) and rectangular squamous MCs (length, 10 μm). There were numerous stomata (∼200 nm) and microparticles (20-200 nm) on the surface of the PVS MCs. In conclusion, this study presents the novel finding that the PVS periductium is composed of squamous MCs. These cells tightly line the luminal surface of the PVS tissue, including PNs, PVs, and small branches of the PVs in the abdominal cavity. These results will help us to understand the physiological roles such as hyaluronan secretion and the fine structure of PVS tissue.
据报道，原血管系统（PVS）具有一层由细胞核呈球形或纺锤形且细胞质丰富的细胞组成的导管周组织。然而，关于这些导管周细胞的了解甚少。在本研究中，我们检查了从大鼠腹部器官表面分离的PVS组织所覆盖细胞的形态特征。通过苏木精和伊红（H&E）染色，我们在原结（PN）、原血管（PV）及其亚单位切片的边界处的基底膜上观察到一层深色细胞核。这些细胞核呈细长形（10 - 14μm）、椭圆形（8 - 10×3 - 4μm）和圆形（5 - 7μm）。PVS组织切片的边界用间皮细胞（MCs）的选择性抗体进行免疫染色。苏木精和伊红染色显示免疫反应区域与扁平细胞重叠。通过扫描电子显微镜，我们进一步鉴定出椭圆形（11×21μm）和长方形鳞状MCs（长度为10μm）。PVS MCs表面有许多气孔（约200nm）和微粒（20 - 200nm）。总之，本研究提出了一个新发现，即PVS导管周组织由鳞状MCs组成。这些细胞紧密排列在PVS组织的腔表面，包括腹腔内的PNs、PVs以及PVs的小分支。这些结果将有助于我们了解透明质酸分泌等生理作用以及PVS组织的精细结构。
