Plant Genome. 2017 Mar;10(1). doi: 10.3835/plantgenome2016.07.0058.
Plant-specific () genes play critical roles in various plant growth and development processes. However, the number and characteristics of genes in soybean [ (L.) Merr.] remain unknown. Here, we identified 90 homologous genes in the soybean genome that phylogenetically clustered into two classes (I and II). The majority of the genes were evenly distributed across all 20 soybean chromosomes, and 77 (81.11%) of them were detected in segmental duplicated regions. Furthermore, the exon-intron organization and motif composition for each were analyzed. A close phylogenetic relationship was identified between the soybean genes and 41 previously reported genes of different plants in the same group, providing insights into their putative functions. Expression analysis indicated that more than half of the genes were expressed, with the two gene classes showing differential tissue expression characteristics; in addition, they were differentially induced by biotic and abiotic stresses. To further explore the functions of genes in soybean, was selected for functional characterization. GmLBD12 was mainly localized to the nucleus and showed high expression in root and seed tissues. Overexpressing in (L.) Heynh resulted in increases in lateral root (LR) number and plant height. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis demonstrated that was induced by drought, salt, cold, indole acetic acid (IAA), abscisic acid (ABA), and salicylic acid SA treatments. This study provides the first comprehensive analysis of the soybean gene family and a valuable foundation for future functional studies of genes.
植物特异性（）基因在各种植物生长和发育过程中发挥着关键作用。然而，大豆[（L.）Merr.]中的基因数量和特征尚不清楚。在这里，我们在大豆基因组中鉴定出 90 个同源基因，这些基因在系统发育上分为两类（I 和 II）。大多数基因均匀分布在大豆的 20 条染色体上，其中 77 个（81.11%）基因位于片段重复区域。此外，我们还分析了每个基因的外显子-内含子组织和基序组成。结果发现，大豆基因与同一组中 41 个先前报道的不同植物基因之间存在密切的系统发育关系，这为它们的潜在功能提供了线索。表达分析表明，超过一半的基因表达，两个基因类别的组织表达特征存在差异；此外，它们还受到生物和非生物胁迫的差异诱导。为了进一步探索大豆基因的功能，我们选择进行功能表征。GmLBD12 主要定位于细胞核，在根和种子组织中表达量较高。在（L.）Heynh 中过表达导致侧根（LR）数量和植物高度增加。定量实时聚合酶链反应（qRT-PCR）分析表明，该基因受到干旱、盐、冷、吲哚乙酸（IAA）、脱落酸（ABA）和水杨酸（SA）处理的诱导。本研究首次对大豆基因家族进行了全面分析，为基因的功能研究提供了有价值的基础。
