Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2022 May 13;23(10):5474. doi: 10.3390/ijms23105474.
Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors involved in various transcriptional regulation processes. We identified a total of 37 genes in ginkgo, and based on gene structure and phylogenetic analysis, the gene family was classified into class I (33, with the largest number of Id genes (16)) and class II (4). The ginkgo gene was also analyzed regarding its chromosomal distributions, gene duplications, promoters, and introns/exons. In addition, gene expression profiling and real-time quantitative PCR analysis showed that the expression of 14 genes differed in six different tissues and three developmental stages. The gene of class II were highly expressed relative to the class I gene in all tissues and developmental stages, while class Id gene were generally at low levels or were not expressed, especially in seed developmental stages. The expression pattern analysis of cold/drought treatment and IAA/ABA hormone treatment showed that abiotic stress treatment could significantly induce the expression of gene, of which class II genes played a key role in stress treatment. Our study provides a solid foundation for further evolutionary and functional analysis of the ginkgo gene family.
侧生器官边界域（LBD）蛋白是一类植物特异性转录因子，参与多种转录调控过程。我们在银杏中总共鉴定到 37 个基因，根据基因结构和系统发育分析，该基因家族被分为 I 类（33 个，其中 Id 基因数量最多（16 个））和 II 类（4 个）。还分析了银杏基因的染色体分布、基因复制、启动子和内含子/外显子。此外，基因表达谱和实时定量 PCR 分析表明，14 个基因在 6 种不同组织和 3 个发育阶段的表达存在差异。在所有组织和发育阶段，II 类基因的表达水平均高于 I 类基因，而 Id 类基因通常处于低水平或不表达，尤其是在种子发育阶段。对冷/干旱处理和 IAA/ABA 激素处理的表达模式分析表明，非生物胁迫处理可显著诱导基因的表达，其中 II 类基因在胁迫处理中发挥关键作用。我们的研究为进一步研究银杏基因家族的进化和功能提供了坚实的基础。
