Van der Auwera P, Matsumoto T, Husson M
Laboratoire d'Investigation Clinique H. J. Tagnon, Service de Médecine, Institut Jules Bordet, Brussels, Belgium.
J Antimicrob Chemother. 1988 Aug;22(2):185-92. doi: 10.1093/jac/22.2.185.
The penetration of several antibiotics into human polymorphonuclear leucocytes was measured with a bioassay. The aminoglycosides (gentamicin, netilmicin), oxacillin and LY146032, a new lipopeptidic antibiotic, had a penetration which was generally less than 60%, whereas new fluoro-quinolones (enoxacin, ciprofloxacin, CI934, Ro236240) and rifamycins (rifampicin, LM427) were concentrated 2.4 to 14.2-fold. The concentration of vancomycin and teicoplanin associated with the neutrophils appeared to be saturable over the range of extracellular concentrations tested (5-20 mg/l). Coumermycin, an inhibitor of DNA-gyrase, was highly concentrated (11.3 to 16.6-fold) within the neutrophils. The penetration of clindamycin and erythromycin was low (0.60- to 1.48-fold).
采用生物测定法测定了几种抗生素在人多形核白细胞中的渗透情况。氨基糖苷类抗生素（庆大霉素、奈替米星）、苯唑西林和新型脂肽类抗生素LY146032的渗透率通常低于60%，而新型氟喹诺酮类抗生素（依诺沙星、环丙沙星、CI934、Ro236240）和利福霉素类抗生素（利福平、LM427）的浓度则提高了2.4至14.2倍。在所测试的细胞外浓度范围（5 - 20毫克/升）内，与中性粒细胞相关的万古霉素和替考拉宁浓度似乎达到了饱和。DNA旋转酶抑制剂香豆霉素在中性粒细胞内高度浓缩（11.3至16.6倍）。克林霉素和红霉素的渗透率较低（0.60至1.48倍）。
