Laboratório Nacional de Luz Síncrotron (LNLS)/Laboratório Nacional de Nanotecnologia (LNNano), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), CEP 13083-970, Caixa Postal 6192, Campinas, SP, Brazil.
Instituto de Química (IQ), Universidade Estadual de Campinas (UNICAMP), CEP 13083-970, Caixa Postal 6154, Campinas, SP, Brazil.
Sci Rep. 2017 May 2;7(1):1326. doi: 10.1038/s41598-017-01209-1.
The rational synthesis of alternative materials is highly demanding due to the outbreak of infectious diseases and resistance to antibiotics. Herein, we report a tailored nanoantibiotic synthesis protocol where the antibiotic binding was optimized on the silver-silica core-shell nanoparticles surface to maximize biological responses. The obtained silver nanoparticles coated with mesoporous silica functionalized with ampicillin presented remarkable antimicrobial effects against susceptible and antibiotic-resistant Escherichia coli. In addition, these structures were not cell-death inducers and different steps of the mitotic cell cycle (prophase, anaphase and metaphase) were clearly identified. The superior biological results were attributed to a proper and tailored synthesis strategy.
由于传染病的爆发和抗生素耐药性的出现，对替代材料的合理合成提出了很高的要求。在此，我们报告了一种定制的纳米抗生素合成方案，其中优化了抗生素结合在银-硅核壳纳米粒子表面上的方式，以最大限度地提高生物响应。所获得的银纳米粒子涂有阿莫西林功能化的介孔硅，对敏感和耐抗生素的大肠杆菌表现出显著的抗菌效果。此外，这些结构不会诱导细胞死亡，并且可以清楚地识别有丝分裂细胞周期的不同阶段（前期、中期和后期）。优异的生物学结果归因于适当和定制的合成策略。
