Petrus P, Edholm D, Rosqvist F, Dahlman I, Sundbom M, Arner P, Rydén M, Risérus U
Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
Department of Surgical Sciences, Uppsala University Hospital, Uppsala University, Uppsala, Sweden.
Int J Obes (Lond). 2017 Aug;41(8):1295-1298. doi: 10.1038/ijo.2017.106. Epub 2017 May 3.
Cardiometabolic diseases are primarily linked to enlarged visceral adipose tissue (VAT). However, some data suggest heterogeneity within the subcutaneous adipose tissue (SAT) depot with potential metabolic differences between the superficial SAT (sSAT) and deep SAT (dSAT) compartments. We aimed to investigate the heterogeneity of these three depots with regard to fatty acid (FA) composition and gene expression. Adipose tissue biopsies were collected from 75 obese women undergoing laparoscopic gastric bypass surgery. FA composition and gene expression were determined with gas chromatography and quantitative real-time-PCR, respectively. Stearoyl CoA desaturase-1 (SCD-1) activity was estimated by product-to-precursor FA ratios. All polyunsaturated FAs (PUFA) with 20 carbons were consistently lower in VAT than either SAT depots, whereas essential PUFA (linoleic acid, 18:2n-6 and α-linolenic acid, 18:3n-3) were similar between all three depots. Lauric and palmitic acid were higher and lower in VAT, respectively. The SCD-1 product palmitoleic acid as well as estimated SCD-1 activity was higher in VAT than SAT. Overall, there was a distinct association pattern between lipid metabolizing genes and individual FAs in VAT. In conclusion, SAT and VAT are two distinct depots with regard to FA composition and expression of key lipogenic genes. However, the small differences between sSAT and dSAT suggest that FA metabolism of SAT is rather homogenous.
心脏代谢疾病主要与内脏脂肪组织（VAT）增大有关。然而，一些数据表明皮下脂肪组织（SAT）库存在异质性，浅表SAT（sSAT）和深部SAT（dSAT）隔室之间可能存在代谢差异。我们旨在研究这三个库在脂肪酸（FA）组成和基因表达方面的异质性。从75名接受腹腔镜胃旁路手术的肥胖女性中采集脂肪组织活检样本。分别用气相色谱法和定量实时PCR法测定FA组成和基因表达。通过产物与前体FA的比率估算硬脂酰辅酶A去饱和酶-1（SCD-1）活性。所有含20个碳的多不饱和脂肪酸（PUFA）在VAT中的含量始终低于任何一个SAT库，而在所有三个库中，必需PUFA（亚油酸，18:2n-6和α-亚麻酸，18:3n-3）含量相似。月桂酸和棕榈酸在VAT中分别较高和较低。VAT中SCD-1产物棕榈油酸以及估算的SCD-1活性高于SAT。总体而言，VAT中脂质代谢基因与单个FA之间存在明显的关联模式。总之，就FA组成和关键生脂基因的表达而言，SAT和VAT是两个不同的库。然而，sSAT和dSAT之间的微小差异表明SAT的FA代谢相当均匀。
