De Re Valli, Repetto Ombretta, Zanussi Stefania, Casarotto Mariateresa, Caggiari Laura, Canzonieri Vincenzo, Cannizzaro Renato
Facility of Bio-Proteomics, Immunopathology and Cancer Biomarkers, IRCCS CRO National Cancer Institute, Via F. Gallini 2, 33081 Aviano, Italy.
Microbiology-Immunology and Virology, IRCCS CRO National Cancer Institute, Aviano, Italy.
Infect Agent Cancer. 2017 Apr 27;12:22. doi: 10.1186/s13027-017-0133-x. eCollection 2017.
() represents a key factor in the etiology of autoimmune atrophic gastritis (AAG), duodenal ulcer (DU) and gastric cancer (GC). The aim of this study was to characterize the differential protein expression of isolated from gastric biopsies of patients affected by either AAG, DU or GC.
The strains were isolated from endoscopic biopsies from the stomach of patients with gastric disease. Protein profiles of were compared by two-dimensional difference in gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS) for the identification of significantly different spots (Student -test, < 0.05).
A total of 47 differentially expressed spots were found between isolated from patients with either DU or AAG diseases and those isolated from patients with GC (Anova < 0.05, log fold change >1.5). These spots corresponded to 35 unique proteins. The identity of 7 protein spots was validated after one-dimensional electrophoresis and MS/MS analyses of excised gel portions. In isolated from DU-patients a significant increase in proteins with antioxidant activity emerged (AroQ, AspA, FldA, Icd, OorA and ScoB), together with a higher content of proteins counteracting the high acid environment (KatA and NapA). In isolated from AAG-patients proteins neutralizing hydrogen concentrations through organic substance metabolic processes decreased (GroL, TrxB and Tuf). In addition, a reduction of bacterial motility (FlhA) was found to be associated with AAG- isolates. In GC- strains it was found an increase in nucleic acid-binding proteins (e.g. DnaG, Tuf, RpoA, RplU) which may be involved in a higher demand of DNA- and protein-related processes.
Our data suggest the presence of specific protein signatures discriminating among isolated from either AAG, DU or GC. Changes in protein expression profiles evaluated by DIGE succeeded in deciphering part of the molecular scenarios associated with the different -related gastric diseases.
（）是自身免疫性萎缩性胃炎（AAG）、十二指肠溃疡（DU）和胃癌（GC）病因中的一个关键因素。本研究的目的是对从AAG、DU或GC患者胃活检组织中分离出的（某种物质，原文未明确）的差异蛋白表达进行表征。
从胃病患者胃的内镜活检组织中分离出（该物质）菌株。通过二维差异凝胶电泳（2D - DIGE）结合质谱（MS）比较（该物质）的蛋白质谱，以鉴定显著不同的斑点（Student检验，P < 0.05）。
在从DU或AAG疾病患者分离出的（该物质）与从GC患者分离出的（该物质）之间共发现47个差异表达斑点（方差分析P < 0.05，对数倍数变化>1.5）。这些斑点对应于35种独特蛋白质。对切下的凝胶部分进行一维电泳和串联质谱分析后，验证了7个蛋白质斑点的身份。在从DU患者分离出的（该物质）中，具有抗氧化活性的蛋白质（AroQ、AspA、FldA、Icd、OorA和ScoB）显著增加，同时抵抗高酸环境的蛋白质（KatA和NapA）含量也更高。在从AAG患者分离出的（该物质）中，通过有机物质代谢过程中和氢浓度的蛋白质（GroL、TrxB和Tuf）减少。此外，发现细菌运动性降低（FlhA）与AAG分离株有关。在GC菌株中，发现核酸结合蛋白（如DnaG、Tuf、RpoA、RplU）增加，这可能与对DNA和蛋白质相关过程的更高需求有关。
我们的数据表明，存在区分从AAG、DU或GC分离出的（该物质）的特定蛋白质特征。通过DIGE评估的蛋白质表达谱变化成功地解读了与不同（该物质）相关胃部疾病相关的部分分子情况。
