Oefinger P E, Loo S H, Gander R M
Department of Pathology, University of Texas Medical School, Houston 77030.
J Clin Microbiol. 1988 Oct;26(10):2195-9. doi: 10.1128/jcm.26.10.2195-2199.1988.
Conventional culture tubes were used in a modification of the spin-amplified adsorption procedure for recovery of herpes simplex virus (HSV) from clinical specimens. The sensitivity of isolation of HSV from 864 specimens adsorbed by the spin-amplified method was 100% (127 of 127), compared with 88.2% (112 of 127) for stationary-phase-adsorbed specimens. Cytopathic effect developed more rapidly in 32.1% (36 of 112) of isolates adsorbed by spin amplification than in those adsorbed by stationary means. In a separate quantitative study, cultures of HSV type 1 adsorbed by spin amplification yielded higher antigen levels and greater cytopathic effect than stationary-phase-adsorbed cultures. Cells grown in conventional tissue culture tubes may be used in a spin-amplified adsorption for rapid detection and increased sensitivity of HSV isolation.
在对从临床标本中回收单纯疱疹病毒（HSV）的旋转扩增吸附程序进行的一项改进中，使用了传统的培养管。通过旋转扩增方法吸附的864份标本中HSV分离的敏感性为100%（127份中的127份），相比之下，固定相吸附标本的敏感性为88.2%（127份中的112份）。通过旋转扩增吸附的分离株中有32.1%（112份中的36份）比通过固定方法吸附的分离株更快出现细胞病变效应。在另一项定量研究中，通过旋转扩增吸附的1型HSV培养物比固定相吸附的培养物产生更高的抗原水平和更大的细胞病变效应。在传统组织培养管中生长的细胞可用于旋转扩增吸附，以实现HSV分离的快速检测并提高敏感性。
