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用于检测人巨细胞病毒免疫球蛋白G抗体的微粒酶免疫测定法

Microparticle enzyme immunoassay for determination of immunoglobulin G antibodies to human cytomegalovirus.

作者信息

Duverlie G, Roussel C, Driencourt M, Orfila J

机构信息

Laboratoire de Virologie, Centre Hospitalier Universitaire, Amiens, France.

出版信息

J Clin Microbiol. 1988 Oct;26(10):2229-30. doi: 10.1128/jcm.26.10.2229-2230.1988.

Abstract

A microparticle enzyme immunoassay (LEIA) that uses passive latex agglutination was used to detect cytomegalovirus immunoglobulin G antibodies in 495 serum samples. LEIA was in excellent concordance with latex agglutination (97%) and an enzyme-linked immunosorbent assay (98.3%). The sensitivity and specificity of LEIA compared with those of the enzyme-linked immunosorbent assay were 100 and 92.5%, respectively.

摘要

采用被动乳胶凝集法的微粒酶免疫测定法(LEIA)用于检测495份血清样本中的巨细胞病毒免疫球蛋白G抗体。LEIA与乳胶凝集法(一致性为97%)和酶联免疫吸附测定法(一致性为98.3%)具有高度一致性。与酶联免疫吸附测定法相比,LEIA的灵敏度和特异性分别为100%和92.5%。

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