超声处理与单克隆抗体联合用于检测离心培养物中早期和晚期巨细胞病毒抗原
Combined use of sonication and monoclonal antibodies for the detection of early and late cytomegalovirus antigens in centrifugation cultures.
作者信息
Agha S A, Coleman J C, Selwyn S, Mahmoud L A, Abd-Elaal A M
机构信息
Department of Medical Microbiology, Charing Cross and Westminster Medical School, London, U.K.
出版信息
J Virol Methods. 1988 Oct;22(1):41-50. doi: 10.1016/0166-0934(88)90086-9.
A total of 157 clinical specimens was inoculated into shell vials and conventional tube cell cultures containing confluent monolayers of human embryonic lung fibroblasts (HELF). Of 31 clinical cytomegalovirus (CMV) isolates, 30 specimens (96.8%) were positive by the immunofluorescence method on centrifugation vial cultures (CVC-IF), whereas the cytopathic effects (CPE) of CMV were detected in only 14 specimens (45.2%) in conventional tube cell cultures (CCC), P less than 0.001 and in 22 specimens (70.9%) in centrifugation vial cultures (CVC-P), P less than 0.1. Significantly more fluorescent foci were detected in centrifugation cultures inoculated with sonicated urine samples (P less than 0.001). CVC-P is more sensitive than CCC for the diagnosis of CMV (P less than 0.05), and a highly significant difference was observed when we compared the mean day to initial detection of CPE (P less than 0.001). For optimal detection of CMV, both CVC-IF and CVC-P should be used for the laboratory diagnosis of this virus infection.
总共157份临床标本被接种到含有汇合的人胚肺成纤维细胞（HELF）单层的空斑试验瓶和传统试管细胞培养物中。在31株临床巨细胞病毒（CMV）分离株中，30份标本（96.8%）通过离心瓶培养免疫荧光法（CVC-IF）呈阳性，而在传统试管细胞培养（CCC）中仅14份标本（45.2%）检测到CMV的细胞病变效应（CPE），P<0.001，在离心瓶培养（CVC-P）中有22份标本（70.9%）检测到CPE，P<0.1。在用超声处理的尿液样本接种的离心培养物中检测到的荧光灶明显更多（P<0.001）。CVC-P在诊断CMV方面比CCC更敏感（P<0.05），并且在比较CPE初始检测的平均天数时观察到高度显著差异（P<0.001）。为了最佳地检测CMV，CVC-IF和CVC-P都应用于该病毒感染的实验室诊断。
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