Leroy Adrien, Ribeiro Sofia, Grossiord Carole, Alves Antoine, Vestberg Robert H, Salles Vincent, Brunon Céline, Gritsch Kerstin, Grosgogeat Brigitte, Bayon Yves
Laboratoire des Multimatériaux et Interfaces, UMR 5615 CNRS-Université Lyon 1, Université de Lyon, 43 bd du 11 Novembre 1918, Villeurbanne, Cedex 69622, France.
Medtronic-Sofradim Production, 116 Avenue du Formans, Trévoux, 01600, France.
J Mater Sci Mater Med. 2017 Jun;28(6):87. doi: 10.1007/s10856-017-5894-7. Epub 2017 May 3.
The integration and evolution of implantable medical devices made of bioresorbable polymers and used for temporary biomedical applications are crucial criteria in the success of a therapy and means of follow-up after implantation are needed. The objective of this work is to develop and evaluate a method based on microscopic Fourier Transform InfraRed spectroscopy (FTIR) mappings to monitor the degradation of such polymers on tissue explant sections, after implantation. This technique provided information on their location and on both their composition and crystallinity, which is directly linked to their state of degradation induced predominantly by chain scissions. An in vitro study was first performed on poly(L-lactic acid) (PLLA) meshes to validate the procedure and the assumption that changes observed on FTIR spectra are indeed a consequence of degradation. Then, mappings of in vivo degraded PLLA meshes were realized to follow up their degradation and to better visualize their degradation mechanisms. This work further warrants its translation to medical implants made of copolymers of lactic acid and to other polyesters.
由生物可吸收聚合物制成并用于临时生物医学应用的植入式医疗设备的整合与演进，是治疗成功的关键标准，且植入后需要后续跟进手段。这项工作的目标是开发并评估一种基于微观傅里叶变换红外光谱（FTIR）映射的方法，以监测此类聚合物在植入后组织外植体切片上的降解情况。该技术提供了有关它们的位置以及组成和结晶度的信息，而这直接与主要由链断裂引起的降解状态相关。首先对聚（L-乳酸）（PLLA）网片进行了体外研究，以验证该程序以及FTIR光谱上观察到的变化确实是降解结果的假设。然后，对体内降解的PLLA网片进行映射，以跟踪其降解过程并更好地可视化其降解机制。这项工作进一步保证了其可转化应用于由乳酸共聚物制成的医疗植入物以及其他聚酯。
