Key Laboratory of Eco-environment-related Polymer Materials, Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University , Lanzhou 730070, China.
School of Basic Medical Sciences, Gansu University of Chinese Medicine , Lanzhou 730000, China.
ACS Appl Mater Interfaces. 2017 May 24;9(20):16756-16766. doi: 10.1021/acsami.6b16575. Epub 2017 May 15.
Inspired by marine mussel adhesive proteins, polymers with catechol side groups have been extensively explored in industrial and academic research. Here, Pluronic L-31 alcoholate ions were used as the initiator to prepare a series of polypeptide-Pluronic-polypeptide triblock copolymers via ring-opening polymerization of l-DOPA-N-carboxyanhydride (DOPA-NCA), l-arginine-NCA (Arg-NCA), l-cysteine-NCA (Cys-NCA), and ε-N-acryloyl lysine-NCA (Ac-Lys-NCA). These copolymers demonstrated good biodegradability, biocompatibility, and thermoresponsive properties. Adhesion tests using porcine skin and bone as adherends demonstrated lap-shear adhesion strengths up to 106 kPa and tensile adhesion strengths up to 675 kPa. The antibleeding activity and tissue adhesive ability were evaluated using a rat model. These polypeptide-Pluronic copolymer glues showed superior hemostatic properties and superior effects in wound healing and osteotomy gaps. Complete healing of skin incisions and remodeling of osteotomy gaps were observed in all rats after 14 and 60 days, respectively. These copolymers have potential uses as tissue adhesives, antibleeding, and tissue engineering materials.
受海洋贻贝类黏附蛋白的启发，带有儿茶酚侧基的聚合物在工业和学术研究中得到了广泛的探索。在这里，Pluronic L-31 醇盐离子被用作引发剂，通过 l-DOPA-N-羧酸酐（DOPA-NCA）、l-精氨酸-N-羧酸酐（Arg-NCA）、l-半胱氨酸-N-羧酸酐（Cys-NCA）和 ε-N-丙烯酰赖氨酰-N-羧酸酐（Ac-Lys-NCA）的开环聚合，制备了一系列多肽-Pluronic-多肽三嵌段共聚物。这些共聚物表现出良好的生物降解性、生物相容性和温敏性。使用猪皮和骨作为附着体的粘附测试表明，搭接剪切粘附强度高达 106 kPa，拉伸粘附强度高达 675 kPa。使用大鼠模型评估了止血活性和组织黏附能力。这些多肽-Pluronic 共聚物胶表现出优异的止血性能，在伤口愈合和骨切开间隙方面具有更好的效果。所有大鼠在 14 天和 60 天后分别观察到皮肤切口完全愈合和骨切开间隙重塑。这些共聚物有望用作组织粘合剂、止血剂和组织工程材料。
