Lu Dedai, Li Yunfei, Wang Xiangya, Li Ting'e, Zhang Yongyong, Guo Hongyun, Sun Shaobo, Wang Xiaoqi, Zhang Yongdong, Lei Ziqiang
Key Laboratory of Eco-environment-related Polymer Materials Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China.
J Mater Chem B. 2018 Dec 7;6(45):7511-7520. doi: 10.1039/c8tb01015c. Epub 2018 Oct 31.
A series of hyperbranched, thermo-responsive and mussel-inspired polypeptides were synthesized and used for surgical adhesion, hemostasis and interventional embolization. These polypeptides showed excellent tissue-adhesive properties according to adhesive strength tests on porcine skin and bone in vitro, where the maximum lap-shear adhesion strength on porcine skin was 114.5 kPa and the maximum tensile adhesion strength on bone was 786 kPa. In vivo animal experiments indicated that these polypeptides exhibit superior hemostatic properties and healing effects in skin incisions and osteotomy gap; the skin incision healing and osteotomy gap remodeling were completed in all rats after 14 and 60 days, respectively. In vivo evaluation of the embolization ability of these polypeptides was performed on rabbit kidney models, resulting in successful occlusion of renal arteries, which led to gross ischemic changes in the embolized kidneys up to 16 days. A trial embolization procedure on H22 tumor-bearing rat models also confirmed the gelability of these polypeptides in tumor arteries, which might cause damage to embolized tumors. Therefore, these polypeptides are expected to be good candidates as surgical tissue adhesives, antibleeding materials, and effective embolic materials.
合成了一系列超支化、热响应性且受贻贝启发的多肽，并将其用于手术粘连、止血和介入栓塞。根据在猪皮肤和骨上的体外粘附强度测试，这些多肽表现出优异的组织粘附性能，其中在猪皮肤上的最大搭接剪切粘附强度为114.5 kPa，在骨上的最大拉伸粘附强度为786 kPa。体内动物实验表明，这些多肽在皮肤切口和截骨间隙中表现出优异的止血性能和愈合效果；分别在14天和60天后，所有大鼠的皮肤切口愈合和截骨间隙重塑均完成。在兔肾模型上对这些多肽的栓塞能力进行了体内评估，结果成功闭塞了肾动脉，导致栓塞的肾脏出现严重缺血变化，持续长达16天。在荷H22肿瘤大鼠模型上进行的栓塞试验程序也证实了这些多肽在肿瘤动脉中的凝胶化能力，这可能会对栓塞的肿瘤造成损伤。因此，这些多肽有望成为良好的手术组织粘合剂、止血材料和有效的栓塞材料候选物。
