Huang Bo, Li Zhou, Ai Jihui, Zhu Lixia, Li Yufeng, Jin Lei, Zhang Hanwang
Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology Wuhan, People's Republic of China.
Int J Clin Exp Pathol. 2014 Apr 15;7(5):2273-82. eCollection 2014.
This study measured the antioxidant activity of follicular fluid (FF) in infertile patients and assessed its possible correlation between ovarian stimulation and pregnancy outcomes. Samples from 191 infertile patients undergoing in vitro fertilization-embryo transfer (IVF-ET) were determined by α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging, reducing power, superoxide radical scavenging, β-Carotene bleaching assay, ferrothiocyanate and thiobarbituric acid assays. The comparison between a positive IVF outcome and FF's antioxidant activity was also studied. The results showed FF had strong antioxidant activity, which equated to common antioxidants Vc and BHT (100 μg/mL). Patients with endometriosis had less efficient antioxidant activity in FF than that of patients with tubal occlusion or polycystic ovary syndrome. In conclusion, this study detected, for the first time, the antioxidant activity of FF from patients undergoing an IVF and the FF exhibited strong antioxidant activity.
本研究测定了不孕患者卵泡液（FF）的抗氧化活性，并评估了其在卵巢刺激与妊娠结局之间可能存在的相关性。对191例行体外受精-胚胎移植（IVF-ET）的不孕患者的样本进行了α-二苯基-β-苦味酰基自由基（DPPH）清除、还原能力、超氧自由基清除、β-胡萝卜素漂白测定、硫氰酸铁和硫代巴比妥酸测定。还研究了IVF阳性结果与FF抗氧化活性之间的比较。结果显示FF具有较强的抗氧化活性，相当于常见抗氧化剂维生素C和丁基羟基甲苯（100μg/mL）。子宫内膜异位症患者FF中的抗氧化活性低于输卵管阻塞或多囊卵巢综合征患者。总之，本研究首次检测到接受IVF患者FF的抗氧化活性，且FF表现出较强的抗氧化活性。
