Chasapis Christos T, Andreini Claudia, Georgiopolou Athanasia K, Stefanidou Maria E, Vlamis-Gardikas Alexios
Institute of Chemical Engineering Sciences (ICE-HT), Foundation for Research and Technology, Hellas (FORTH), Stadiou St., Platani, 26504, Patras, Greece.
Magnetic Resonance Center (CERM) Department of Chemistry, University of Florence, 50019, Sesto Fiorentino, Italy.
Arch Microbiol. 2017 Oct;199(8):1141-1149. doi: 10.1007/s00203-017-1385-y. Epub 2017 May 6.
Tetrahymena thermophila (T. thermophila) is a ciliated protozoon that can detect freshwater pollution by heavy metals ("whole-cell biosensor"). This work employed a systematic bioinformatics approach to predict and analyze the metalloproteome of T. thermophila for the essential Zn, Cu and the non-essential Cd. 3784 metal-binding proteins were identified compared to the 456 annotated so far in UniProt. The localization, functional classification, and the functionally enriched network of the newly identified metalloproteome are presented. Cd toxicity could be explained in terms of the metal replacing Cu and especially Zn in MAPKs, transporters and antioxidant enzymes. The predicted results for Cd toxicity and responses reflect those observed experimentally in different organisms after their exposure to Cd.
嗜热四膜虫是一种纤毛原生动物，能够检测重金属对淡水的污染（“全细胞生物传感器”）。这项工作采用系统的生物信息学方法，对嗜热四膜虫中必需的锌、铜以及非必需的镉的金属蛋白质组进行预测和分析。与目前在UniProt中注释的456个相比，共鉴定出3784个金属结合蛋白。本文展示了新鉴定的金属蛋白质组的定位、功能分类以及功能富集网络。镉的毒性可以通过金属取代丝裂原活化蛋白激酶（MAPKs）、转运蛋白和抗氧化酶中的铜，尤其是锌来解释。镉毒性和反应的预测结果反映了不同生物体在接触镉后实验中观察到的结果。
