Falahee M B, Weiss R B, O'Connor M, Doonan S, Gesteland R F, Atkins J F
Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.
J Biol Chem. 1988 Dec 5;263(34):18099-103.
External suppressors, sufS, of a -1 frameshift mutant cause ribosomes to shift into the -1 frame when reading the sequence CAG GGA GUG. The resulting product is not Gln-Gly-Val but Gln-Gly-Ser with Ser being encoded by the underlined AGU. The alleles investigated are approximately 2% efficient in causing frameshifting. Two other suppressors, hopR and hopE of the same -1 frameshift mutant, cause some ribosomes reading the sequence GUG UG to decode a single amino acid, Val, from the five nucleotides. The possibility is considered that peptidyl-tRNA(Val) dissociates from the mRNA, but re-pairs in a triplet manner after the mRNA slips forward by two bases.
-1移码突变体的外部抑制基因sufS，使核糖体在读取序列CAG GGA GUG时移至-1读框。产生的产物不是Gln-Gly-Val，而是Gln-Gly-Ser，其中Ser由下划线标注的AGU编码。所研究的等位基因在导致移码方面的效率约为2%。同一-1移码突变体的另外两个抑制基因hopR和hopE，使一些读取序列GUG UG的核糖体从五个核苷酸中解码出单个氨基酸Val。研究认为存在这样一种可能性，即肽基-tRNA(Val)从mRNA上解离，但在mRNA向前滑动两个碱基后以三联体方式重新配对。
