马钱科（Loganiaceae）植物叶提取物及其馏分的一氧化氮抑制活性
NITRIC OXIDE INHIBITORY ACTIVITY OF (LOGANIACEAE) LEAF EXTRACTS AND FRACTIONS.
作者信息
Ai Isa, Jp Dzoyem, Sa Adebayo, Mm Suleiman, Jn Eloff
机构信息
Phytomedicine Programme, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.
Permanent Address, Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria, Nigeria.
出版信息
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):22-26. doi: 10.21010/ajtcam.v13i6.5. eCollection 2016.
BACKGROUND
The study was aimed at determining the anti-inflammatory activity of fractions and extracts obtained from leaves on a mediator of inflammation nitric oxide (NO).
MATERIALS AND METHODS
Leaves were extracted with acetone and separated into fractions with different polarities by solvent-solvent fractionation. The Griess assay was used to determine the nitric oxide (NO) inhibitory activity. Cellular toxicity was determined by "using the MTT reduction assay".
RESULTS
With the exception of the ethyl acetate fraction which had an IC >750 μg/mL, all extracts and fractions had significant nitric oxide-inhibitory activity. The most active being the water fraction, chloroform fraction and the dichloromethane/methanol extracts with IC values of 88.43 μg/mL, 96.72 μg/mL and 115.62 μg/mL, respectively. The extracts and fractions had low cytotoxicity on macrophage U937 cell lines.
CONCLUSION
Extracts and fractions of leaves may be promising sources of natural anti-inflammatory agents. Findings obtained from this study showed that leaves possess promising anti-inflammatory action and could be used in the treatment of inflammation-related conditions.
背景
本研究旨在确定从树叶中获得的提取物和馏分对炎症介质一氧化氮（NO）的抗炎活性。
材料与方法
用丙酮提取树叶，并通过溶剂-溶剂分级分离成不同极性的馏分。采用格里斯试剂法测定一氧化氮（NO）抑制活性。通过“使用MTT还原试验”测定细胞毒性。
结果
除乙酸乙酯馏分的IC>750μg/mL外，所有提取物和馏分均具有显著的一氧化氮抑制活性。活性最强的是水馏分、氯仿馏分和二氯甲烷/甲醇提取物，IC值分别为88.43μg/mL、96.72μg/mL和115.62μg/mL。提取物和馏分对巨噬细胞U937细胞系的细胞毒性较低。
结论
树叶的提取物和馏分可能是天然抗炎剂的有前途的来源。本研究的结果表明，树叶具有有前途的抗炎作用，可用于治疗炎症相关疾病。
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