麦地那薄荷对大鼠肝脏中致癌物代谢酶的调节作用
MODULATION OF CARCINOGEN-METABOLIZING ENZYME BY MADINAH MINT () IN RAT LIVER.
作者信息
Al-Malki Abdulrahman L, Barbour Elie K, Ea Huwait, Moselhy Said S, Choudhry Hani
机构信息
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O Box 80203, Jeddah, Saudi Arabia.
Bioactive Natural Products Research Group, KAU.
出版信息
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):32-37. doi: 10.21010/ajtcam.v13i6.7. eCollection 2016.
BACKGROUND
The present study was undertaken to assess whether boiling water mint extract (BWME) modulates the cytochrome P mixed function oxidase system.
MATERIALS AND METHODS
Male albino rats were randomly divided into two groups, comprising 12 animals each. The first group served as control, whereas the second was maintained on BWME (10 % w/v) as its sole drinking liquid for six weeks. Liver microsomal were separated and subjected for phase I and II enzymes (cytochrome P450 mixed function oxidase) analysis.
RESULTS
The results obtained showed that, BWME caused a significant elevation in the activity of epoxide hydrolase (p<0.001) when compared with the control. However, glutathione S-transferase and glucuronosyl transferase activities were significantly decreased (p<0.001 and p<0.01) respectively compared with control. The mutagenic activity of N-nitrosopiperidine was lower in the mint-treated hepatic microsomal compared with the controls.
CONCLUSION
It can be concluded that BWME has the potential to suppress the activity of cytochrome enzymes involved in the bio-activation of chemical carcinogen; hence may display chemo preventive activity.
背景
本研究旨在评估薄荷水煮提取物（BWME）是否能调节细胞色素P混合功能氧化酶系统。
材料与方法
将雄性白化病大鼠随机分为两组，每组12只。第一组作为对照组，第二组以BWME（10% w/v）作为唯一饮液，持续六周。分离肝微粒体并进行I相和II相酶（细胞色素P450混合功能氧化酶）分析。
结果
所得结果表明，与对照组相比，BWME导致环氧化物水解酶活性显著升高（p<0.001）。然而，与对照组相比，谷胱甘肽S-转移酶和葡糖醛酸基转移酶活性分别显著降低（p<0.001和p<0.01）。与对照组相比，经薄荷处理的肝微粒体中N-亚硝基哌啶的诱变活性较低。
结论
可以得出结论，BWME有可能抑制参与化学致癌物生物活化的细胞色素酶的活性；因此可能具有化学预防活性。
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