Rasooli I, Shayegh S, Astaneh Sda
Department of Biology, Shahed University, Tehran, Iran.
Int J Dent Hyg. 2009 Aug;7(3):196-203. doi: 10.1111/j.1601-5037.2009.00389.x.
To assess the antimicrobial effects of Mentha spicata and Eucalyptus camaldulensis essential oils and chlorhexidine against Streptococcus mutans and Streptococcus pyogenes, with a particular focus on in vitro and in vivo biofilm formation.
The essential oils were analysed by gas chromatography (GC) and GC-mass spectrometry. In vitro and in vivo antimicrobial and biofilm preventing activities of the oils were studied.
Fifteen and 21 compounds were identified in the essential oils of M. spicata and E. camaldulensis respectively. Minimal bactericidal concentrations (MBC) of the M. spicata and E. camaldulensis oils were found to be 4 and 2 mg ml(-1), and those of chlorhexidine (2%) were 8 and 1 mg ml(-1) for both S. mutans and S. pyogenes respectively. Decimal reduction time of S. mutans by M. spicata and E. camaldulensis oils at their MBC levels was 2.8 min, while that of cholrhexidine was 12.8 min. D-value of S. pyogenes exposed to the MBC levels of M. spicata and E. camaldulensis oils and of chlorhexidine were 4.3, 3.6 and 2.8 min respectively. Antibacterial and in vivo biofilm preventive efficacies of all the concentrations of eucalyptus oil were significantly (P < 0.001) higher than that of M. spicata oil and chlorhexidine. In conclusion, essential oils are capable of affecting biofilm formation.
The essential oils from E. camaldulensis and M. spicata significantly retard biofilm formation that can contribute to the development of novel anticaries treatments.
评估留兰香和赤桉精油以及氯己定对变形链球菌和化脓性链球菌的抗菌作用，尤其关注体外和体内生物膜的形成。
采用气相色谱（GC）和GC-质谱联用仪分析精油。研究了精油的体外和体内抗菌及生物膜预防活性。
留兰香和赤桉精油分别鉴定出15种和21种化合物。留兰香和赤桉精油对变形链球菌和化脓性链球菌的最低杀菌浓度（MBC）分别为4和2 mg/ml，而2%氯己定对这两种菌的MBC分别为8和1 mg/ml。留兰香和赤桉精油在MBC水平下对变形链球菌的D值为2.8分钟，而氯己定的D值为12.8分钟。化脓性链球菌暴露于留兰香和赤桉精油以及氯己定MBC水平下的D值分别为4.3、3.6和2.8分钟。所有浓度的桉叶油的抗菌和体内生物膜预防效果均显著高于留兰香油和氯己定（P < 0.001）。总之，精油能够影响生物膜的形成。
赤桉和留兰香的精油能显著抑制生物膜形成，这有助于开发新型防龋治疗方法。
