Bakr Riham Omar, Mohamed Shaza Abd El Halim, Ayoub Nahla
Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA).
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University (Girls).
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):135-143. doi: 10.21010/ajtcam.v13i6.19. eCollection 2016.
L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS).
Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay.
A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC of 12.1, 30.9, 11.7 and 19.5 μg/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1).
This study provides a better understanding of the chemistry of that announces itself as a promising cytotoxic agent.
L（菊科）是在西奈半岛野生生长的最具吸引力的植物之一，其植物化学成分尚未得到充分研究。本研究首次利用液相色谱（LC）结合电喷雾电离（ESI）串联质谱（MS/MS）对甲醇提取物地上部分的酚类成分进行了深入表征。
利用LC-HRESI-MS-MS研究酚类成分。使用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）法对四种人类癌细胞系（Hep-G2；肝癌细胞、MCF-7；乳腺腺癌细胞、HCT-116；结肠癌细胞和HELA；宫颈癌细胞）进行细胞毒性活性评估。利用细胞病变效应抑制试验检测抗病毒活性。
在负离子和正离子模式下，共初步鉴定出61种化合物（21种酚酸及其衍生物、31种黄酮醇和9种黄酮）。与作为参考药物的阿霉素相比，对Hep-G2、MCF-7、HCT-116和HELA细胞系显示出优异的结果，IC分别为12.1、30.9、11.7和19.5μg/mL。对甲型肝炎病毒（HAV）的抗病毒活性较弱，对单纯疱疹病毒1型（HSV 1）无影响。
本研究对L的化学性质有了更好的了解，L显示出作为一种有前景的细胞毒性剂的潜力。
