Al-Malki Abdulrahman L, Moselhy Said S
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia'.
Bioactive Natural Products Research Group, KAU.
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):149-154. doi: 10.21010/ajtcam.v13i6.21. eCollection 2016.
The objective of this study was to investigate the fatty acids profiling in diabetic rats induced by sterptozocine (STZ) and their response to administration of lutein and carnitine.
Ninety male albino rats were divided into 6 groups as follows: Normal control. The remaining rats were injected a single dose of STZ (65 mg /kg bw) for induction of diabetes. Diabetic rats were grouped as: GP II: (Untreated): GP III: Rats were given orally with L-lutein (100 mg/kg bw).GP IV: Rats were given carnitine (30 μg/kg) . GP V: Rats were given carnitine and lutein GP VI were given metformin (100mg/kg bw/d) for 6 weeks.
Treatment of diabetic rats with lutein, L-carnitine, combined decreased the levels of glucose, HA1C compared with untreated diabetic (p<0.001). Administration of L-lutein, carnitine, combined to normal rats significantly decreased the levels of myristic, palmitice, palmitoleic, stearic, linoleic, α-linolenic, arachidic and eicosadienoic when compared with control normal rats (p<0.001).
Abnormalities of fatty acids composition was observed in diabetic rats. Combination treatment with lutein and carnitine could ameliorate deleterious effect induced by STZ and attenuate the changed fatty acid composition.
本研究的目的是调查链脲佐菌素（STZ）诱导的糖尿病大鼠的脂肪酸谱及其对叶黄素和肉碱给药的反应。
90只雄性白化大鼠分为6组，如下：正常对照组。其余大鼠注射单剂量STZ（65mg/kg体重）以诱导糖尿病。糖尿病大鼠分为：第二组（未治疗组）；第三组：大鼠口服L-叶黄素（100mg/kg体重）；第四组：大鼠给予肉碱（30μg/kg）；第五组：大鼠给予肉碱和叶黄素；第六组给予二甲双胍（100mg/kg体重/天），持续6周。
与未治疗的糖尿病大鼠相比，用叶黄素、L-肉碱联合治疗糖尿病大鼠可降低血糖、糖化血红蛋白水平（p<0.001）。与正常对照大鼠相比，给正常大鼠施用L-叶黄素、肉碱联合用药可显著降低肉豆蔻酸、棕榈酸、棕榈油酸、硬脂酸、亚油酸、α-亚麻酸、花生酸和二十碳二烯酸的水平（p<0.001）。
在糖尿病大鼠中观察到脂肪酸组成异常。叶黄素和肉碱联合治疗可改善STZ诱导的有害作用，并减轻脂肪酸组成的变化。
