College of Chemistry, Chemical Engineering and Biotechnology, Donghua University , Shanghai 201620, China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , Shanghai 201620, China.
ACS Appl Mater Interfaces. 2017 May 31;9(21):17786-17798. doi: 10.1021/acsami.7b04488. Epub 2017 May 16.
The construction of safe and stable theranostics is beneficial to realize simultaneous cancer diagnosis and treatment. In this study, bovine serum albumin-gadolinium (BSA-Gd) complexes and MoS nanoflakes (MoS-Gd-BSA) were successfully married as cancer theranostics for dual-modality magnetic resonance (MR)/photoacoustic (PA) imaging and photothermal therapy (PTT). BSA-Gd complexes were prepared by the biomineralization method and then conjugated with MoS nanoflakes via an amide bond. The as-prepared MoS-Gd-BSA possessed a good photostability and photothermal effect. The cytotoxicity assessment and hemolysis assay suggested the excellent biocompatibility of MoS-Gd-BSA. Meanwhile, MoS-Gd-BSA could not only achieve in vivo MR/PA dual-modality imaging of xenograft tumors, but also effectively kill cancer cells in vitro and ablate the xenograft tumors in vivo upon 808 nm laser illumination. The biodistribution and histological evaluations indicated the negligible toxicity of MoS-Gd-BSA both in vitro and in vivo. Thus, our results substantiated the potential of MoS-Gd-BSA for cancer theranostics.
构建安全稳定的治疗策略有利于实现癌症的同时诊断与治疗。在本研究中，牛血清白蛋白-钆（BSA-Gd）复合物和 MoS 纳米片（MoS-Gd-BSA）成功结合作为癌症治疗策略用于磁共振（MR）/光声（PA）成像和光热治疗（PTT）的双模式成像。BSA-Gd 复合物通过生物矿化法制备，然后通过酰胺键与 MoS 纳米片偶联。所制备的 MoS-Gd-BSA 具有良好的光稳定性和光热效应。细胞毒性评估和溶血试验表明 MoS-Gd-BSA 具有优异的生物相容性。同时，MoS-Gd-BSA 不仅可以实现异种移植瘤的体内 MR/PA 双模式成像，而且可以在 808nm 激光照射下有效杀死体外的癌细胞并消融体内的异种移植瘤。生物分布和组织学评价表明 MoS-Gd-BSA 在体外和体内均具有可忽略的毒性。因此，我们的结果证实了 MoS-Gd-BSA 在癌症治疗学中的应用潜力。
