Kawecki Stephanie, Kuleck Gary, Dorsey John H, Leary Christopher, Lum Michelle
Loyola Marymount University, 1 LMU Drive, LSB 101, Los Angeles, CA, 90045, USA.
University of Detroit Mercy, 4001 W. McNichols Road, Detroit, MI, 48221, USA.
Environ Monit Assess. 2017 Jun;189(6):261. doi: 10.1007/s10661-017-5964-9. Epub 2017 May 10.
Screening for the prevalence of antibiotic-resistant bacteria (ARB) was done at the Ballona Creek and Wetlands, an urban-impacted wetland system in Los Angeles, California. The goals were (1) to assess the overall prevalence of ARB, and (2) compare differences in ARB abundance and the types of antibiotic resistance (AR) among the following sample types: lagoon water from Del Rey Lagoon, urban runoff from Ballona Creek, and water from the Ballona Wetlands (tidal water flooding in from the adjacent estuary, and ebbing out from the salt marsh). Antibiotic resistance distributions were analyzed using the Kolmogorov-Smirnov test to develop the cumulative frequency of bacteria having resistance of up to eight antibiotics. Distributions from the environmental water samples were compared to unchlorinated secondary effluent from the Hyperion Water Reclamation Plant that was used as comparator samples likely to have an abundance of ARB. As expected, densities of total and ARB were highest in secondary effluent, followed by urban runoff. Samples of water flooding into the wetlands showed similar results to urban runoff; however, a reduction in densities of total and ARB occurred in water ebbing out of the wetlands. During preliminary work to identify ARB species, several bacterial species of relevance to human illness (e.g., Staphylococcus aureus, Enterococcus hirae, Pseudomonas aeruginosa, Aeromonas veronii, Enterobacter cancerogenus, Serratia marcescens, Pseudomonas stutzeri, and Staphylococcus intermedius) were isolated from sampled waters. If wetlands are a sink for ARB, construction and restoration of wetlands can help in the mediation of this human and environmental health concern.
在位于加利福尼亚州洛杉矶市、受城市影响的湿地系统巴洛纳溪及湿地进行了抗生素抗性细菌（ARB）流行情况的筛查。目标是：（1）评估ARB的总体流行情况；（2）比较以下样本类型中ARB丰度及抗生素抗性（AR）类型的差异：德雷泻湖的泻湖水、巴洛纳溪的城市径流以及巴洛纳湿地的水（来自相邻河口的潮水涌入，并从盐沼退去）。使用柯尔莫哥洛夫-斯米尔诺夫检验分析抗生素抗性分布，以得出对多达八种抗生素具有抗性的细菌的累积频率。将环境水样的分布与海波龙水回收厂的未氯化二级出水进行比较，后者用作可能含有大量ARB的对照样本。正如预期的那样，二级出水中总细菌和ARB的密度最高，其次是城市径流。流入湿地的水样结果与城市径流相似；然而，从湿地流出的水中总细菌和ARB的密度有所降低。在鉴定ARB物种的初步工作中，从采样水中分离出了几种与人类疾病相关的细菌物种（例如金黄色葡萄球菌、平脐肠球菌、铜绿假单胞菌、维罗纳气单胞菌、致癌肠杆菌、粘质沙雷氏菌、施氏假单胞菌和中间葡萄球菌）。如果湿地是ARB的汇，那么湿地的建设和恢复有助于缓解这一关乎人类和环境健康的问题。
