• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

通过使用貂肺细胞培养物的空斑小瓶离心试验提高巨细胞病毒快速检测的灵敏度。

Increased sensitivity for rapid detection of cytomegalovirus by shell vial centrifugation assay using mink lung cell cultures.

作者信息

MacKenzie D, McLaren L C

机构信息

Department of Microbiology, University of New Mexico School of Medicine, Albuquerque 87131.

出版信息

J Virol Methods. 1989 Nov;26(2):183-8. doi: 10.1016/0166-0934(89)90147-x.

DOI:10.1016/0166-0934(89)90147-x
PMID:2559101
Abstract

A comparative study was made of various human and non-human cell cultures to determine their sensitivity for cytomegalovirus (CMV) as detected by the production of CMV early antigen using the shell vial centrifugation assay. Mink lung cell cultures, frequently used for detection of herpes simplex virus in clinical specimens, were found to be significantly more sensitive to infection by CMV than other cell cultures tested. Using the shell vial centrifugation assay, the mink lung cell cultures were more sensitive than human diploid fibroblasts for the detection of the Davis strain of human CMV and CMV from clinical specimens.

摘要

进行了一项关于各种人类和非人类细胞培养物的比较研究,以确定它们对巨细胞病毒(CMV)的敏感性,通过使用空斑离心试验检测CMV早期抗原的产生来进行评估。水貂肺细胞培养物常用于临床标本中单纯疱疹病毒的检测,结果发现其对CMV感染的敏感性明显高于其他测试的细胞培养物。使用空斑离心试验,水貂肺细胞培养物在检测人CMV戴维斯株和临床标本中的CMV时比人二倍体成纤维细胞更敏感。

相似文献

1
Increased sensitivity for rapid detection of cytomegalovirus by shell vial centrifugation assay using mink lung cell cultures.通过使用貂肺细胞培养物的空斑小瓶离心试验提高巨细胞病毒快速检测的灵敏度。
J Virol Methods. 1989 Nov;26(2):183-8. doi: 10.1016/0166-0934(89)90147-x.
2
Effect of leukocyte concentration and inoculum volume on the laboratory identification of cytomegalovirus in peripheral blood by the centrifugation culture-antigen detection methodology.白细胞浓度和接种量对采用离心培养-抗原检测法进行外周血巨细胞病毒实验室鉴定的影响。
Arch Pathol Lab Med. 1996 Jan;120(1):53-6.
3
Combined use of sonication and monoclonal antibodies for the detection of early and late cytomegalovirus antigens in centrifugation cultures.超声处理与单克隆抗体联合用于检测离心培养物中早期和晚期巨细胞病毒抗原
J Virol Methods. 1988 Oct;22(1):41-50. doi: 10.1016/0166-0934(88)90086-9.
4
Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.用于检测临床标本中巨细胞病毒的标准管和细胞培养瓶细胞培养技术的比较。
J Clin Microbiol. 1985 Feb;21(2):217-21. doi: 10.1128/jcm.21.2.217-221.1985.
5
Clinical comparison of two assays for rapid detection of cytomegalovirus early nuclear antigen.
Am J Clin Pathol. 1990 Mar;93(3):373-7. doi: 10.1093/ajcp/93.3.373.
6
Clinical experience with cytomegalovirus isolation using conventional cell cultures and early antigen detection in centrifugation-enhanced shell vial cultures.使用传统细胞培养技术分离巨细胞病毒以及在离心增强的空斑培养中进行早期抗原检测的临床经验。
J Clin Microbiol. 1989 Jun;27(6):1159-62. doi: 10.1128/jcm.27.6.1159-1162.1989.
7
Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures.通过空斑试验和传统的试管细胞培养法检测尿液以外标本中的巨细胞病毒感染。
J Clin Microbiol. 1987 May;25(5):755-7. doi: 10.1128/jcm.25.5.755-757.1987.
8
[Rapid diagnosis of infection by cytomegalovirus using early-antigen detection (shell vial)].[使用早期抗原检测(空斑小瓶法)进行巨细胞病毒感染的快速诊断]
Enferm Infecc Microbiol Clin. 1991 Nov;9(9):564-6.
9
Rapid detection of cytomegalovirus in clinical specimens by immunofluorescent staining of shell vial cultures.通过对空斑小室培养物进行免疫荧光染色快速检测临床标本中的巨细胞病毒。
Am J Clin Pathol. 1988 Apr;89(4):528-32. doi: 10.1093/ajcp/89.4.528.
10
Detection of cytomegalovirus by 24-well plate centrifugation assay using a monoclonal antibody to an early nuclear antigen and by conventional cell culture.使用针对早期核抗原的单克隆抗体通过24孔板离心试验以及通过传统细胞培养法检测巨细胞病毒。
J Virol Methods. 1987 Dec;18(4):207-13. doi: 10.1016/0166-0934(87)90082-6.

引用本文的文献

1
Developing a Vaccine against Congenital Cytomegalovirus (CMV) Infection: What Have We Learned from Animal Models? Where Should We Go Next?开发针对先天性巨细胞病毒(CMV)感染的疫苗:我们从动物模型中学到了什么?接下来我们该何去何从?
Future Virol. 2013 Dec;8(12):1161-1182. doi: 10.2217/fvl.13.106.
2
Physical and chemical methods for enhancing rapid detection of viruses and other agents.用于加强病毒及其他病原体快速检测的物理和化学方法。
Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150.
3
Comparison of MRC-5 and U-373MG astrocytoma cells for detection of cytomegalovirus in shell vial centrifugation cultures.
用于检测空斑小瓶离心培养物中巨细胞病毒的MRC-5和U-373MG星形细胞瘤细胞的比较
Eur J Clin Microbiol Infect Dis. 1991 Jul;10(7):569-72. doi: 10.1007/BF01967276.
4
Detection of human cytomegalovirus in clinical specimens by centrifugation culture with a nonhuman cell line.使用非人细胞系通过离心培养法检测临床标本中的人巨细胞病毒。
J Clin Microbiol. 1992 Apr;30(4):1045-8. doi: 10.1128/jcm.30.4.1045-1048.1992.