Wirgart B Z, Grillner L
J Virol Methods. 1986 Aug;14(1):65-9. doi: 10.1016/0166-0934(86)90008-x.
A commercially available monoclonal antibody directed against early cytomegalovirus (CMV) antigen was used for the demonstration of CMV by immunofluorescence (IF) in cell culture within 2 days. The results were compared with the appearance of CMV-specific cytopathogenic effect (CPE). Urine specimens from 31 healthy children in day-care centers were inoculated on human embryonic fibroblasts. In addition, 45 CMV strains that had been stored at -70 degrees C were reinoculated. CMV was detected in 8/31 urine specimens by IF and 7 of these gave a specific CPE at an average of 16 days post-inoculation. One specimen was negative by IF but specific CPE was found at day 13. After reinoculation, CMV was detected in 76% by IF while 44 specimens developed CPE within a 6-week period. Demonstration of early CMV antigen in cell culture was found to be a rapid method for early diagnosis of CMV. Since the conventional cell culture with detection of CPE was more sensitive it may be useful to combine the two methods.
一种针对早期巨细胞病毒（CMV）抗原的市售单克隆抗体被用于在2天内通过免疫荧光（IF）在细胞培养中检测CMV。将结果与CMV特异性细胞病变效应（CPE）的出现情况进行比较。来自日托中心31名健康儿童的尿液标本接种于人胚成纤维细胞。此外，对45株保存在-70℃的CMV毒株进行了重新接种。通过IF在8/31份尿液标本中检测到CMV，其中7份在接种后平均16天出现特异性CPE。1份标本IF检测为阴性，但在第13天发现了特异性CPE。重新接种后，通过IF在76%的标本中检测到CMV，而44份标本在6周内出现了CPE。在细胞培养中检测早期CMV抗原被发现是早期诊断CMV的一种快速方法。由于检测CPE的传统细胞培养更敏感，将这两种方法结合可能会有用。
