Alexandre K, Rooryck J, Galand P
Biology Unit, IRIBHN, Free University of Brussels, Belgium.
J Steroid Biochem. 1988 Dec;31(6):873-5. doi: 10.1016/0022-4731(88)90327-5.
We previously reported on a positive correlation between two effects of estrogen on rat uterus, namely the early increases in cGMP and in water content of the organ suggesting that they were under the control of the same hormone sensitive regulatory process or linked by a cause to effect relationship. Up to now we were unable to find experimental conditions that would dissociate the two responses. In this work, immature Wistar rats were treated with colchicine (50 micrograms/animal) given at the same time as estradiol-17 beta (1 microgram/animal) or with estradiol alone. The experiments showed: (1) that the estradiol induced increase in uterine wet weight that occurs during the first 8 h after hormone injection was completely suppressed in the presence of colchicine indicating that it might depend on an intact microtubular system and (2) that, by contrast, the estrogen-induced increase in uterine cGMP remained unaffected by the colchicine presence. These data allow to conclude that the cGMP response to estradiol can be dissociated from the wet weight response and, therefore, that it is not controlled by the latter. From this and from data in the literature the hypothesis is proposed that the increase in uterine cGMP content might trigger the wet weight response, this possibly through a positive action on some microtubular function.
我们之前报道过雌激素对大鼠子宫的两种作用之间存在正相关，即早期cGMP增加和器官含水量增加，这表明它们受同一激素敏感调节过程的控制，或者存在因果关系。到目前为止，我们尚未找到能使这两种反应分离的实验条件。在这项研究中，对未成熟的Wistar大鼠同时给予秋水仙碱（50微克/只动物）和17β-雌二醇（1微克/只动物），或仅给予雌二醇。实验结果表明：（1）在秋水仙碱存在的情况下，激素注射后最初8小时内雌二醇诱导的子宫湿重增加被完全抑制，这表明该增加可能依赖于完整的微管系统；（2）相反，雌激素诱导的子宫cGMP增加不受秋水仙碱存在的影响。这些数据表明，对雌二醇的cGMP反应可与湿重反应分离，因此不受后者控制。基于此以及文献中的数据，我们提出假说：子宫cGMP含量的增加可能通过对某些微管功能的正向作用触发湿重反应。
