Qian Bing-Jun, Wu Chou-Fei, Lu Man-Man, Xu Wei, Jing Pu
Research Center for Food Safety and Nutrition, Key Lab of Urban Agriculture (South), Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Collaborative Innovation Center of Green Pesticide, College of Life Science, Huzhou University, Huzhou 313000, PR China.
Food Chem. 2017 Oct 1;232:545-551. doi: 10.1016/j.foodchem.2017.04.010. Epub 2017 Apr 6.
Studies have enabled a molecular understanding of the anthocyanin copigmentation phenomenon over several decades. However, the effect of combinations of, or even supramolecular assemblies of, anthocyanins with other phenols and/or metal ions on their antioxidative activity was unclear. In this study, anthocyanin complexes of cyanidin-3-diglucoside-5-glucoside (CY3D5G), rutin and Mg(II)/Fe(III) were constructed, analyzed, and evaluated for their antioxidant effects. The CY3D5G-rutin-Fe(III) exhibited supramolecular properties via visible, CD and FTIR spectra among complexes. The interaction of CY3D5G-rutin, CY3D5G-rutin-Mg(II), or CY3D5G-rutin-Fe(III) was synergistic (P<0.05) in the ORAC assay. On cellular ROS levels, the median effective concentration of the CY3D5G-rutin-Mg(II) was 7.76μmol QE/L and exhibited a synergistic interaction (CI=0.67, P<0.05), whereas the CY3D5G-rutin-Fe(III) (CI=0.79, P=0.074) was additive. The results indicate that the antioxidant properties were affected by the molecular combination. Additionally, Fe(III) might exhibit a negative effect, since the CY3D5G-Fe(III) required a greater concentration than CY3D5G to achieve the same effect on cells.
几十年来，多项研究使人们对花青素共色素沉着现象有了分子层面的理解。然而，花青素与其他酚类物质和/或金属离子的组合甚至超分子组装对其抗氧化活性的影响尚不清楚。在本研究中，构建、分析并评估了矢车菊素 - 3 - 二葡萄糖苷 - 5 - 葡萄糖苷（CY3D5G）、芦丁与Mg(II)/Fe(III)形成的花青素复合物的抗氧化作用。通过可见光谱、圆二色光谱和傅里叶变换红外光谱分析发现，CY3D5G - 芦丁 - Fe(III)在复合物中表现出超分子特性。在氧自由基吸收能力（ORAC）测定中，CY3D5G - 芦丁、CY3D5G - 芦丁 - Mg(II)或CY3D5G - 芦丁 - Fe(III)之间的相互作用具有协同性（P<0.05）。在细胞活性氧水平方面，CY3D5G - 芦丁 - Mg(II)的半数有效浓度为7.76μmol QE/L，表现出协同相互作用（协同指数CI = 0.67，P<0.05），而CY3D5G - 芦丁 - Fe(III)（CI = 0.79，P = 0.074）则表现为相加作用。结果表明，抗氧化性能受分子组合的影响。此外，Fe(III)可能具有负面影响，因为CY3D5G - Fe(III)对细胞产生相同效果所需的浓度高于CY3D5G。
