Zhang Nan, Jiao Shunshan, Jing Pu
Shanghai Food Safety and Engineering Technology Research Center, Key Lab of Urban Agriculture Ministry of Agriculture, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Antioxidants (Basel). 2021 Jun 8;10(6):930. doi: 10.3390/antiox10060930.
Many studies have demonstrated that cabbages possess various biological activities, and our previous studies confirmed that cyanidin-3-diglucoside-5-glucoside (CY3D5G), the major core of red cabbage anthocyanins, exhibited in vitro antioxidant activity. This study further investigated the protective effects of CY3D5G derivative from red cabbage juice (RCJ) on oxidative stress and lifespan in cells and , green cabbage juice (GCJ) was used as control. RCJ rather than GCJ significantly improved cell viability and decreased lactate dehydrogenase release in HO-induced caco-2 cells. RCJ significantly increased survival during oxidative and heat stress and mean lifespan in by 171.63% and 31.64%, and 28.16%, respectively, while GCJ treatment showed no significant effects ( < 0.05). These results might be attributed to significantly ( < 0.05) higher contents of total phenolics, ascorbic acid, glucosinolates, and anthocyanins in RCJ compared to those in GCJ. Additionally, both of them decreased autofluorescence and reproductive capacity, increased body length, but did not alter the intracellular ROS level. Prolonged lifespan by RCJ might require heat-shock transcription factor pathway, sirtuin signaling, and calmodulin kinase II pathway, independent of insulin/insulin-like growth factor-1 signaling pathway. RCJ showed promising antioxidant properties in caco-2 cells and , which provided more information on the health benefits of cabbage.
许多研究表明，卷心菜具有多种生物活性，我们之前的研究证实，红甘蓝花青素的主要核心成分矢车菊素 - 3 - 二葡萄糖苷 - 5 - 葡萄糖苷（CY3D5G）具有体外抗氧化活性。本研究进一步探讨了红甘蓝汁（RCJ）中的CY3D5G衍生物对细胞氧化应激和寿命的保护作用，并以绿甘蓝汁（GCJ）作为对照。在过氧化氢诱导的caco - 2细胞中，RCJ而非GCJ显著提高了细胞活力并降低了乳酸脱氢酶的释放。RCJ显著提高了秀丽隐杆线虫在氧化应激和热应激下的存活率以及平均寿命，分别提高了171.63%、31.64%和28.16%，而GCJ处理则无显著影响（P < 0.05）。这些结果可能归因于与GCJ相比，RCJ中总酚类、抗坏血酸、硫代葡萄糖苷和花青素的含量显著更高（P < 0.05）。此外，它们都降低了自发荧光和繁殖能力，增加了体长，但没有改变细胞内活性氧水平。RCJ延长寿命可能需要热休克转录因子途径、沉默调节蛋白信号通路和钙调蛋白激酶II途径，而不依赖于胰岛素/胰岛素样生长因子 - 1信号通路。RCJ在caco - 2细胞和秀丽隐杆线虫中显示出有前景的抗氧化特性，这为卷心菜的健康益处提供了更多信息。
