Kotani Masato, Katagiri Fumihiko, Hirai Tsuyoshi, Kagawa Jiro, Tanaka Issei
a Asahina Shinryoujo , Fujieda , Japan.
b Department of Internal Medicine , Fujieda Municipal General Hospital , Fujieda , Japan.
Gynecol Endocrinol. 2017 Oct;33(10):819-821. doi: 10.1080/09513590.2017.1320380. Epub 2017 May 11.
The kisspeptin is a neuropeptide to play physiological roles in regulating gonadotropin-releasing hormone secretion in the hypothalamus. In human plasma, the kisspeptin concentration is measured, but gonadotropin-releasing hormone is not. This study aims to understand the physiological roles of the circulating kisspeptin in lactational amenorrhea in humans because prolactin reduces the kisspeptin expression and luteinizing hormone secretion resulting in anovulations in rodent brains. Plasma kisspeptin levels were measured in 11 subjects in lactational amenorrhea and in four cases with pathological amenorrhea by different etiologies for comparison using the enzyme immunoassay specific for human kisspeptin. The plasma kisspeptin levels in the 11 women with lactational amenorrhea were 15.2 ± 2.5 fmol/mL (mean ± SD) which were not significantly different as compared with 16.5 ± 4.8 fmol/mL (mean ± SD) in four age-matched women with menstrual cycles as we reported previously. In the four cases with pathological amenorrhea, their plasma kisspeptin levels were from 5.8 to 13.7 fmol/mL. This study demonstrated that the plasma kisspeptin levels were not totally reduced in lactational or pathological amenorrhea. These results suggest the physiological roles of the circulating kisspeptin are different from the role in the brain.
kisspeptin是一种神经肽，在下丘脑调节促性腺激素释放激素分泌过程中发挥生理作用。在人体血浆中，可以检测到kisspeptin的浓度，但促性腺激素释放激素则无法检测。本研究旨在了解循环中的kisspeptin在人类哺乳期闭经中的生理作用，因为催乳素会降低kisspeptin的表达以及黄体生成素的分泌，从而导致啮齿动物大脑出现无排卵现象。通过使用针对人类kisspeptin的酶免疫测定法，对11名哺乳期闭经患者和4例不同病因的病理性闭经患者的血浆kisspeptin水平进行了测量，以便进行比较。11名哺乳期闭经女性的血浆kisspeptin水平为15.2±2.5 fmol/mL（平均值±标准差），与我们之前报道的4名月经周期正常的年龄匹配女性的16.5±4.8 fmol/mL（平均值±标准差）相比，差异无统计学意义。在4例病理性闭经患者中，其血浆kisspeptin水平为5.8至13.7 fmol/mL。本研究表明，哺乳期或病理性闭经时血浆kisspeptin水平并未完全降低。这些结果表明，循环中的kisspeptin的生理作用与在大脑中的作用不同。
