Farman Cindy, Schuetz Chris, Lorget Florence, Bantseev Vladimir, Calise David, Thackaberry Evan A
1 Genentech, Inc, South San Francisco, CA, USA.
2 Farman Pathology, Reno, NV, USA.
Vet Pathol. 2017 Sep;54(5):870-876. doi: 10.1177/0300985817705169. Epub 2017 May 11.
Historically, it was thought that lens protein was sequestered, and injury to the lens capsule causing release of lens material into the eye would always result in ocular inflammation. Currently, it is believed that lens antigens are recognized as self, subject to normal T-cell tolerance. Three different single-dose intravitreal injection/implantation studies of 4 different test materials, ranging from 4 to 6 weeks in length, were performed in New Zealand White rabbits. The test materials included polymer microspheres, polymer rods, a solvent, and a hydrogel. Intravitreal injection/implantation procedures were performed on day 1, and indirect ophthalmoscopy and slit-lamp biomicroscopy examinations were performed by board-certified veterinary ophthalmologists periodically throughout the course of each study. None of the affected animals received corticosteroids or other immunomodulatory agents during the course of the studies. Four rabbits had perforation of the posterior lens capsule during the injection/implantation procedure on day 1, visible on clinical ophthalmic examination as lens capsule alterations described as "lens hits" and/or incipient posterior cataracts. Findings on slit-lamp biomicroscopy examination were limited to vitreous cells in 2 of the animals, although not centered on the area of lens capsule disturbance. Histologically, there was no evidence of inflammation in association with extruded lens protein material in any of the affected eyes. These results indicate that iatrogenic damage to the lens capsule during aseptically performed intravitreal injections/implantations does not appear to induce inflammation in rabbits.
从历史上看，人们认为晶状体蛋白是被隔离的，晶状体囊膜受损导致晶状体物质释放到眼内总会引发眼部炎症。目前，人们认为晶状体抗原被识别为自身抗原，处于正常的T细胞耐受状态。在新西兰白兔身上进行了三项不同的单剂量玻璃体内注射/植入研究，使用了4种不同的测试材料，研究时长为4至6周。测试材料包括聚合物微球、聚合物棒、一种溶剂和一种水凝胶。在第1天进行玻璃体内注射/植入操作，在每项研究过程中，由具备委员会认证的兽医眼科医生定期进行间接检眼镜检查和裂隙灯生物显微镜检查。在研究过程中，没有一只受影响的动物接受皮质类固醇或其他免疫调节药物治疗。4只兔子在第1天的注射/植入操作过程中出现了晶状体后囊膜穿孔，在临床眼科检查中可见晶状体囊膜改变，描述为“晶状体撞击”和/或早期后发性白内障。裂隙灯生物显微镜检查结果仅在2只动物的玻璃体中发现细胞，尽管并非集中在晶状体囊膜干扰区域。组织学检查显示，在任何一只受影响的眼睛中，均没有与挤出的晶状体蛋白物质相关的炎症证据。这些结果表明，在无菌进行的玻璃体内注射/植入过程中对晶状体囊膜造成的医源性损伤似乎不会在兔子中诱发炎症。
