Clark J D, Hill S M, Phillips A D
Department of Electron Microscopy, Queen Elizabeth Hospital for Children, London, England.
J Med Virol. 1988 Nov;26(3):289-99. doi: 10.1002/jmv.1890260309.
Rotavirus is a common cause of diarrhoea both in the community and in the hospital. Because of this, it may be difficult to determine whether crossinfection has occurred in the hospital, an important finding as review of hygienic techniques and ward closure may be indicated. We therefore investigated the use of Polyacrylamide gel electrophoresis (PAGE) of the rotavirus RNA genome as a means of distinguishing between rotavirus strains in order to assess its role in the evaluation of apparent hospital-acquired rotavirus diarrhoea. Suspected examples of hospital-acquired rotavirus gastroenteritis were studied on an infectious diseases ward and a general infant ward. PAGE analysis demonstrated that crossinfection had not occurred on the infectious diseases ward, even though this was indicated clinically; a single source outbreak involving 11 patients was confirmed on the general infant ward, as all cases showed an identical rotavirus electropherotype. Following ward closure an endemic rotavirus electropherotype was detected, which affected 17 patients over a 3-month period. Electrophoresis of rotavirus RNA is a useful and practical technique in the analysis of hospital-acquired gastroenteritis and can indicate appropriate clinical action.
轮状病毒是社区和医院腹泻的常见病因。因此，在医院中可能难以确定是否发生了交叉感染，而这一重要发现可能提示需要对卫生技术进行审查并关闭病房。因此，我们研究了轮状病毒RNA基因组的聚丙烯酰胺凝胶电泳（PAGE），以此作为区分轮状病毒株的一种方法，以评估其在评估明显的医院获得性轮状病毒腹泻中的作用。在传染病病房和普通婴儿病房对疑似医院获得性轮状病毒胃肠炎病例进行了研究。PAGE分析表明，传染病病房未发生交叉感染，尽管临床显示有交叉感染迹象；普通婴儿病房证实发生了一起涉及11名患者的单一来源暴发，因为所有病例的轮状病毒电泳图谱均相同。病房关闭后，检测到一种地方性轮状病毒电泳图谱，在3个月内影响了17名患者。轮状病毒RNA电泳是分析医院获得性胃肠炎的一种有用且实用的技术，可指示适当的临床行动。
