Keskin Cumali, Aktepe Necmettin, Yükselten Yunus, Sunguroglu Asuman, Boğa Mehmet
Mardin Artuklu University, School of Health, Department of Nutrition and Dietetics, Mardin, Turkey.
Mardin Artuklu University, School of Health, Department of Nursing, Mardin, Turkey.
Iran J Pharm Res. 2017 Winter;16(1):210-220.
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the and the benefits of seed methanol extracts.
本研究调查了奥氏蔷薇花、果实和种子甲醇提取物的抗氧化、抗癌、抗胆碱酯酶、抗基因毒性活性及酚类成分。通过高效液相色谱法测定甲醇提取物中原儿茶酸、儿茶素、咖啡酸和丁香酸的含量。发现种子提取物的总酚含量高于果实和花提取物。花和种子甲醇提取物的DPPH自由基清除活性与作为对照的丁基羟基甲苯表现出相近的活性。在三种提取物中，只有花甲醇提取物对HeLa和NRK-52E细胞系表现出显著的细胞毒性活性。此外，种子甲醇提取物表现出乙酰胆碱酯酶和丁酰胆碱酯酶抑制活性。在25和50μg/mL浓度下观察到最高的抗基因毒性作用。在本研究中，提取物表现出强大的抗氧化和抗基因毒性作用。种子提取物比果实和花提取物更有效。我们的结果表明，提取物的抗氧化和抗基因毒性作用取决于其酚类成分。进一步的研究应评估种子甲醇提取物的……及其益处。
