Eruygur Nuraniye, Ucar Esra, Akpulat Hüseyin Aşkın, Shahsavari Keyhan, Safavi Seyed Mehdi, Kahrizi Danial
Department of Pharmacognosy, Faculty of Pharmacy, University of Selcuk, Konya, Turkey.
Department of Crop and Animal Production, Sivas Vocational School, Cumhuriyet University, Sivas, Turkey.
Mol Biol Rep. 2019 Apr;46(2):2121-2129. doi: 10.1007/s11033-019-04664-3. Epub 2019 Feb 14.
Hypericum lydium Boiss. is a perennial plant of the Hypericaceae family, which has been used in particular to treat depression. The aim of this study was to determine in vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (α-glucosidase/α-amylase) and Tyrosinase inhibitor activity of methanol and water extracts of H. lydium. Also, gene expression has been evaluated in the shoot and root by microarray technology. So, in general, the purpose of this study is to study the active molecules such as antioxidant, antimicrobial, antidiabetic, enzymes and genes in the plant, which is the first to be reported. The experiments were conducted in a completely randomized design with three replications. In addition, gene expression was compared in the shoot and root parts. Expression profiling was carried out by microarrays. According to the results, the highest chemical components were determined in methanol extract rather than water extract. There was a difference between the obtained components. While the highest antioxidant activity was determined from the methanol extract of plant herbs for DPPH Free Radical Scavenging Activity, antioxidant activity was the same in both methanol and water extracts using the ABTS method. The methanol extract demonstrated stronger anticholinesterase (AChE and BChE) and α-amylase inhibition activity. This study was complemented by the detection of antioxidant activity and some enzyme inhibition activity in the methanol extract. Microarray showed 10,784 genes had significantly different expression in root and shoot. There was a positive effect of methanol extract in respect of different activities compared to the water extract. Gene expression showed that the number of expressed genes in the root was greater than the shoot.
吕底亚金丝桃（Hypericum lydium Boiss.）是金丝桃科的一种多年生植物，尤其被用于治疗抑郁症。本研究的目的是测定吕底亚金丝桃甲醇提取物和水提取物的体外抗氧化、抗菌活性、抗胆碱酯酶（乙酰胆碱酯酶（AChE）/丁酰胆碱酯酶（BChE））、抗糖尿病活性（α-葡萄糖苷酶/α-淀粉酶）以及酪氨酸酶抑制活性。此外，还通过微阵列技术评估了该植物地上部分和根部的基因表达。所以，总体而言，本研究的目的是研究该植物中的抗氧化、抗菌、抗糖尿病、酶和基因等活性分子，这在首次被报道。实验采用完全随机设计，重复三次。此外，还比较了地上部分和根部的基因表达。通过微阵列进行表达谱分析。结果显示，甲醇提取物中的化学成分含量高于水提取物。所获得的成分存在差异。在DPPH自由基清除活性方面，植物药甲醇提取物的抗氧化活性最高，而使用ABTS法时，甲醇提取物和水提取物的抗氧化活性相同。甲醇提取物表现出更强的抗胆碱酯酶（AChE和BChE）和α-淀粉酶抑制活性。本研究通过检测甲醇提取物中的抗氧化活性和一些酶抑制活性得到了补充。微阵列显示10784个基因在根部和地上部分的表达存在显著差异。与水提取物相比，甲醇提取物在不同活性方面具有积极作用。基因表达显示根部的表达基因数量多于地上部分。
